Detailed information of TA system TA105489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 17489..18139
• Replicon: chromosome
• Accession: NZ_CP029355
• Organism: Azospirillum thermophilum strain CFH 70021

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: DEW08_RS20960
• Protein ID: WP_109331106.1
• Coordinates: 17489..17677 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: DEW08_RS20965
• Protein ID: WP_109330999.1
• Coordinates: 17738..18139 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DEW08_RS20950	15241..16194	-	954	WP_211107305.1	alpha/beta fold hydrolase	-
DEW08_RS20955	16582..16899	+	318	WP_109330996.1	hypothetical protein	-
DEW08_RS20960	17489..17677	+	189	WP_109331106.1	type II toxin-antitoxin system HicA family toxin	Toxin
DEW08_RS20965	17738..18139	+	402	WP_109330999.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
DEW08_RS20970	18270..18563	-	294	WP_109331001.1	hypothetical protein	-
DEW08_RS20975	18994..19398	-	405	Protein_18	transposase	-
DEW08_RS20980	19763..20383	-	621	WP_109331004.1	response regulator transcription factor	-
DEW08_RS20985	20373..21986	-	1614	WP_109331006.1	MASE1 domain-containing protein	-
DEW08_RS20990	22154..23008	+	855	WP_109331008.1	universal stress protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (6-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6849.88 Da
• Isoelectric Point: 11.6048

>T105489 WP_109331106.1 NZ_CP029355:17489-17677 [Azospirillum thermophilum]
MSSREVIALLQADGWREVAQTGSHKQFKHPSKTGRVTVPSPVRDLPPGTLKSIERQSGVKLR

Nucleotide

• Length: 189 bp

>T105489 NZ_CP029355:17489-17677 [Azospirillum thermophilum]
ATGAGCAGCCGGGAGGTGATCGCCCTCCTCCAAGCCGACGGATGGCGCGAAGTCGCCCAGACAGGCTCCCACAAGCAGTT
CAAACACCCCAGCAAGACAGGCCGCGTCACAGTCCCCAGCCCGGTCCGAGACCTCCCACCCGGAACCCTCAAGTCGATCG
AACGACAGTCCGGTGTGAAGCTTCGGTAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 13815.64 Da
• Isoelectric Point: 4.5034

>AT105489 WP_109330999.1 NZ_CP029355:17738-18139 [Azospirillum thermophilum]
MVAAYYPLILERTADGLHGSFPDVPGCIAFGASKAELVANAEAALALHFAGIVEDGDPIPAPSDLDAAAADPEVAEAGRL
LVRAELPGKAVRINVTLEEGLLAAIDAEARRRDTSRSGFLAAAARRELAQARQ

Nucleotide

• Length: 402 bp

>AT105489 NZ_CP029355:17738-18139 [Azospirillum thermophilum]
ATGGTTGCCGCCTACTATCCGCTGATCCTCGAGCGCACCGCCGATGGCCTGCACGGATCCTTCCCGGACGTGCCGGGCTG
CATCGCCTTCGGCGCCAGCAAAGCCGAGCTGGTCGCCAACGCCGAGGCCGCCCTCGCCCTCCACTTCGCCGGCATCGTCG
AGGACGGCGACCCCATCCCCGCTCCGTCCGACCTCGACGCCGCCGCGGCCGATCCCGAGGTCGCGGAGGCCGGCCGCCTG
CTGGTCCGTGCCGAGCTGCCGGGCAAGGCGGTGCGGATCAACGTCACGCTCGAGGAGGGATTGCTCGCGGCGATCGACGC
CGAGGCCCGCCGCCGCGACACCTCGCGATCCGGCTTCCTCGCCGCCGCCGCCCGGCGCGAGCTCGCCCAGGCTCGGCAGT
AA