Detailed information of TA system TA105461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4694615..4695201
• Replicon: chromosome
• Accession: NZ_CP029328
• Organism: Escherichia coli strain A1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: AOY52_RS22925
• Protein ID: WP_050574907.1
• Coordinates: 4694971..4695201 (-)
• Length: 77 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: AOY52_RS22920
• Protein ID: WP_000593555.1
• Coordinates: 4694615..4694974 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY52_RS22895 (4690747)	4690747..4691691	+	945	WP_000947068.1	nickel ABC transporter permease subunit NikB	-
AOY52_RS22900 (4691688)	4691688..4692521	+	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
AOY52_RS22905 (4692521)	4692521..4693285	+	765	WP_001136231.1	nickel import ATP-binding protein NikD	-
AOY52_RS22910 (4693282)	4693282..4694088	+	807	WP_000173630.1	nickel import ATP-binding protein NikE	-
AOY52_RS22915 (4694094)	4694094..4694495	+	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
AOY52_RS22920 (4694615)	4694615..4694974	-	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AOY52_RS22925 (4694971)	4694971..4695201	-	231	WP_050574907.1	type II toxin-antitoxin system HicA family toxin	Toxin
AOY52_RS22930 (4695305)	4695305..4696429	-	1125	WP_001314210.1	ABC transporter permease	-
AOY52_RS22935 (4696429)	4696429..4699164	-	2736	WP_024227743.1	ribosome-associated ATPase/putative transporter RbbA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (15-65)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 77 a.a.
• Molecular weight: 8518.92 Da
• Isoelectric Point: 9.3693

>T105461 WP_050574907.1 NZ_CP029328:c4695201-4694971 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRRSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Nucleotide

• Length: 231 bp

>T105461 NZ_CP029328:c4695201-4694971 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGAGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT105461 WP_000593555.1 NZ_CP029328:c4694974-4694615 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT105461 NZ_CP029328:c4694974-4694615 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LKZ6