Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4673108..4673908 Replicon chromosome
Accession NZ_CP029328
Organism Escherichia coli strain A1

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag AOY52_RS22800 Protein ID WP_000342449.1
Coordinates 4673108..4673635 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag AOY52_RS22805 Protein ID WP_001277108.1
Coordinates 4673642..4673908 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY52_RS22775 (4668183) 4668183..4668950 - 768 WP_000082110.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
AOY52_RS22780 (4668947) 4668947..4670224 - 1278 WP_000803782.1 branched chain amino acid ABC transporter permease LivM -
AOY52_RS22785 (4670221) 4670221..4671147 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
AOY52_RS22790 (4671195) 4671195..4672304 - 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
AOY52_RS22795 (4672728) 4672728..4673111 + 384 WP_000778771.1 aspartate 1-decarboxylase autocleavage activator PanM -
AOY52_RS22800 (4673108) 4673108..4673635 - 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
AOY52_RS22805 (4673642) 4673642..4673908 - 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
AOY52_RS22810 (4674058) 4674058..4675161 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
AOY52_RS22815 (4675432) 4675432..4676286 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
AOY52_RS22820 (4676531) 4676531..4677589 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
AOY52_RS22825 (4677582) 4677582..4678250 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T105460 WP_000342449.1 NZ_CP029328:c4673635-4673108 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T105460 NZ_CP029328:c4673635-4673108 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT105460 WP_001277108.1 NZ_CP029328:c4673908-4673642 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT105460 NZ_CP029328:c4673908-4673642 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References