Detailed information of TA system TA105454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3515958..3516583
• Replicon: chromosome
• Accession: NZ_CP029328
• Organism: Escherichia coli strain A1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AOY52_RS17090
• Protein ID: WP_000911330.1
• Coordinates: 3515958..3516356 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: AOY52_RS17095
• Protein ID: WP_000450524.1
• Coordinates: 3516356..3516583 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY52_RS17070 (3511836)	3511836..3512036	+	201	WP_000383836.1	YpfN family protein	-
AOY52_RS17075 (3512146)	3512146..3512844	-	699	WP_000679812.1	esterase	-
AOY52_RS17080 (3512918)	3512918..3514933	-	2016	WP_047086710.1	tRNA cytosine(34) acetyltransferase TmcA	-
AOY52_RS17085 (3514948)	3514948..3515811	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
AOY52_RS17090 (3515958)	3515958..3516356	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
AOY52_RS17095 (3516356)	3516356..3516583	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
AOY52_RS17100 (3516737)	3516737..3517450	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
AOY52_RS17105 (3517663)	3517663..3518697	-	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
AOY52_RS17110 (3518714)	3518714..3519592	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
AOY52_RS17115 (3519738)	3519738..3520310	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
AOY52_RS17120 (3520310)	3520310..3520780	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T105454 WP_000911330.1 NZ_CP029328:c3516356-3515958 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T105454 NZ_CP029328:c3516356-3515958 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT105454 WP_000450524.1 NZ_CP029328:c3516583-3516356 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT105454 NZ_CP029328:c3516583-3516356 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33