Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1268209..1268827 Replicon chromosome
Accession NZ_CP029328
Organism Escherichia coli strain A1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY52_RS05945 Protein ID WP_001291435.1
Coordinates 1268209..1268427 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY52_RS05950 Protein ID WP_000344800.1
Coordinates 1268453..1268827 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY52_RS05910 (1263499) 1263499..1264071 + 573 WP_000779826.1 YbaY family lipoprotein -
AOY52_RS05915 (1264102) 1264102..1264413 - 312 WP_000409911.1 MGMT family protein -
AOY52_RS05925 (1264792) 1264792..1265145 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY52_RS05930 (1265187) 1265187..1266737 - 1551 WP_089633415.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY52_RS05935 (1266901) 1266901..1267371 - 471 WP_000136192.1 YlaC family protein -
AOY52_RS05940 (1267487) 1267487..1268038 - 552 WP_000102564.1 maltose O-acetyltransferase -
AOY52_RS05945 (1268209) 1268209..1268427 - 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY52_RS05950 (1268453) 1268453..1268827 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY52_RS05955 (1269373) 1269373..1272522 - 3150 WP_001132462.1 efflux RND transporter permease AcrB -
AOY52_RS05960 (1272545) 1272545..1273738 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T105440 WP_001291435.1 NZ_CP029328:c1268427-1268209 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T105440 NZ_CP029328:c1268427-1268209 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT105440 WP_000344800.1 NZ_CP029328:c1268827-1268453 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT105440 NZ_CP029328:c1268827-1268453 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References