Detailed information of TA system TA105439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1233913..1234750
• Replicon: chromosome
• Accession: NZ_CP029328
• Organism: Escherichia coli strain A1

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: AOY52_RS05775
• Protein ID: WP_000227784.1
• Coordinates: 1233913..1234455 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: AOY52_RS05780
• Protein ID: WP_001297137.1
• Coordinates: 1234439..1234750 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY52_RS05755 (1229452)	1229452..1230363	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
AOY52_RS05760 (1230531)	1230531..1231022	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
AOY52_RS05765 (1231150)	1231150..1232514	-	1365	WP_001000974.1	MFS transporter	-
AOY52_RS05770 (1232922)	1232922..1233857	+	936	WP_001377877.1	tetratricopeptide repeat protein	-
AOY52_RS05775 (1233913)	1233913..1234455	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
AOY52_RS05780 (1234439)	1234439..1234750	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
AOY52_RS05785 (1234935)	1234935..1235825	-	891	WP_000971336.1	heme o synthase	-
AOY52_RS05790 (1235837)	1235837..1236166	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AOY52_RS05795 (1236166)	1236166..1236780	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AOY52_RS05800 (1236770)	1236770..1238761	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AOY52_RS05805 (1238783)	1238783..1239730	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T105439 WP_000227784.1 NZ_CP029328:c1234455-1233913 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T105439 NZ_CP029328:c1234455-1233913 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT105439 WP_001297137.1 NZ_CP029328:c1234750-1234439 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT105439 NZ_CP029328:c1234750-1234439 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3