Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1351509..1352127 Replicon chromosome
Accession NZ_CP029327
Organism Escherichia coli strain A37

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY90_RS06300 Protein ID WP_001291435.1
Coordinates 1351509..1351727 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY90_RS06305 Protein ID WP_000344800.1
Coordinates 1351753..1352127 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY90_RS06265 (1346796) 1346796..1347368 + 573 WP_000779831.1 YbaY family lipoprotein -
AOY90_RS06270 (1347399) 1347399..1347710 - 312 WP_000409908.1 MGMT family protein -
AOY90_RS06280 (1348089) 1348089..1348442 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY90_RS06285 (1348484) 1348484..1350034 - 1551 WP_001298569.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY90_RS06290 (1350198) 1350198..1350668 - 471 WP_000136192.1 YlaC family protein -
AOY90_RS06295 (1350784) 1350784..1351335 - 552 WP_000102539.1 maltose O-acetyltransferase -
AOY90_RS06300 (1351509) 1351509..1351727 - 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY90_RS06305 (1351753) 1351753..1352127 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY90_RS06310 (1352671) 1352671..1355820 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
AOY90_RS06315 (1355843) 1355843..1357036 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T105413 WP_001291435.1 NZ_CP029327:c1351727-1351509 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T105413 NZ_CP029327:c1351727-1351509 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT105413 WP_000344800.1 NZ_CP029327:c1352127-1351753 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT105413 NZ_CP029327:c1352127-1351753 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References