Detailed information of TA system TA105411

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 826643..827193
• Replicon: chromosome
• Accession: NZ_CP029327
• Organism: Escherichia coli strain A37

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A8H8ZN44
• Locus tag: AOY90_RS03825
• Protein ID: WP_001160969.1
• Coordinates: 826879..827193 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: AOY90_RS03820
• Protein ID: WP_000125566.1
• Coordinates: 826643..826876 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY90_RS03795 (821716)	821716..822003	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
AOY90_RS03800 (822062)	822062..823393	+	1332	WP_001183207.1	MFS transporter	-
AOY90_RS03805 (823501)	823501..824031	+	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
AOY90_RS03810 (824024)	824024..825886	+	1863	WP_097752025.1	glutathione-regulated potassium-efflux system protein KefC	-
AOY90_RS03815 (826078)	826078..826557	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
AOY90_RS03820 (826643)	826643..826876	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
AOY90_RS03825 (826879)	826879..827193	+	315	WP_001160969.1	CcdB family protein	Toxin
AOY90_RS03830 (827190)	827190..828038	-	849	WP_000257182.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
AOY90_RS03835 (828045)	828045..828422	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
AOY90_RS03840 (828425)	828425..829246	-	822	WP_240728715.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
AOY90_RS03845 (829243)	829243..830232	-	990	WP_000241229.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
AOY90_RS03850 (830232)	830232..831518	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11681.65 Da
• Isoelectric Point: 9.0851

>T105411 WP_001160969.1 NZ_CP029327:826879-827193 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIVGEINRRIVIPLTPIERFSRIRPPERLNPILLPVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T105411 NZ_CP029327:826879-827193 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTGTTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACCGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACAAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT105411 WP_000125566.1 NZ_CP029327:826643-826876 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT105411 NZ_CP029327:826643-826876 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9