Detailed information of TA system TA105404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 2767350..2767969
• Replicon: chromosome
• Accession: NZ_CP029289
• Organism: Acidianus brierleyi strain DSM 1651

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DFR85_RS30765
• Protein ID: WP_281351097.1
• Coordinates: 2767607..2767969 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: DFR85_RS30760
• Protein ID: WP_110271568.1
• Coordinates: 2767350..2767607 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DFR85_RS30740 (DFR85_14930)	2762628..2763212	-	585	WP_110271565.1	IS607-like element ISSis7 family transposase	-
DFR85_RS30745 (DFR85_14935)	2763239..2764051	+	813	WP_110271566.1	APC family permease	-
DFR85_RS30750 (DFR85_14940)	2764266..2766260	-	1995	WP_110271567.1	acetate--CoA ligase	-
DFR85_RS30755 (DFR85_14945)	2766353..2766916	+	564	Protein_2958	acetate uptake transporter	-
DFR85_RS30760 (DFR85_14950)	2767350..2767607	+	258	WP_110271568.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DFR85_RS30765 (DFR85_14955)	2767607..2767969	+	363	WP_281351097.1	type II toxin-antitoxin system VapC family toxin	Toxin
DFR85_RS32055	2768438..2769115	-	678	WP_246253086.1	IS110 family transposase	-
DFR85_RS32175	2769079..2769213	-	135	WP_281351043.1	hypothetical protein	-
DFR85_RS32060	2769201..2769581	-	381	WP_246252935.1	transposase	-
DFR85_RS30775 (DFR85_14970)	2770248..2771024	+	777	WP_110271570.1	enoyl-CoA hydratase/isomerase family protein	-
DFR85_RS30780 (DFR85_14975)	2771027..2772712	-	1686	WP_110271571.1	AMP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2746904..2769581	22677

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-119)

Antitoxin

• SpoVT-AbrB domain (4-46)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 14082.43 Da
• Isoelectric Point: 4.9658

>T105404 WP_281351097.1 NZ_CP029289:2767607-2767969 [Acidianus brierleyi]
IFLDANFLIYLNLGVKEIEEVYLKLLREESLALDPLVLDEVIYVSRKKYNVKFKDTIEFLDEIVLQNSILFSITSNEYKR
AKDFMINYSLKPSDSLHVAVMLNNSIKKILTEDKDFDKIK

Nucleotide

• Length: 363 bp

>T105404 NZ_CP029289:2767607-2767969 [Acidianus brierleyi]
ATATTTTTAGATGCAAATTTTCTCATATACTTAAACTTAGGTGTAAAGGAGATAGAAGAAGTCTATCTTAAACTATTAAG
GGAAGAGAGCTTAGCCTTAGATCCTTTAGTGTTAGATGAAGTTATCTATGTTTCTAGAAAGAAATATAATGTTAAATTTA
AAGATACTATAGAATTTCTTGATGAAATAGTGCTACAGAATTCAATACTCTTCTCAATAACTTCTAACGAATATAAAAGA
GCCAAAGATTTCATGATAAATTATTCTTTAAAACCTTCCGATTCTCTTCATGTAGCTGTTATGTTAAATAATTCTATTAA
AAAGATTTTAACTGAGGATAAAGATTTTGATAAAATTAAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9800.42 Da
• Isoelectric Point: 5.9006

>AT105404 WP_110271568.1 NZ_CP029289:2767350-2767607 [Acidianus brierleyi]
MTLRIEVGKKGYIIIPKNIRDLIGIKEGDTIILNVENGRIILEPERKVNINDIIRKLNEHEDKISYAKKIKLGDLAQTSL
EEEFE

Nucleotide

• Length: 258 bp

>AT105404 NZ_CP029289:2767350-2767607 [Acidianus brierleyi]
TTGACTCTAAGAATCGAAGTAGGTAAAAAAGGTTATATAATAATTCCAAAAAATATTAGGGATCTTATAGGAATTAAGGA
AGGAGATACCATAATTCTAAATGTCGAAAATGGAAGAATAATTTTGGAACCAGAGAGAAAAGTAAATATTAATGATATTA
TTAGGAAACTTAACGAACATGAGGATAAAATATCTTATGCTAAGAAAATAAAGTTGGGTGATTTAGCTCAAACAAGCTTA
GAAGAGGAGTTTGAATAA