Detailed information of TA system TA105366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Phd(antitoxin)
• Location: 67855..68399
• Replicon: chromosome
• Accession: NZ_CP029263
• Organism: Xanthomonas sp. MLO165

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: DGN21_RS00245
• Protein ID: WP_228960823.1
• Coordinates: 68130..68399 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2N7V9L6
• Locus tag: DGN21_RS00240
• Protein ID: WP_016902922.1
• Coordinates: 67855..68112 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DGN21_RS00225 (DGN21_00240)	62966..64471	-	1506	WP_160955038.1	ribonuclease H-like domain-containing protein	-
DGN21_RS00230 (DGN21_00245)	64468..66963	-	2496	WP_215825055.1	DEAD/DEAH box helicase	-
DGN21_RS00235 (DGN21_00250)	67137..67799	+	663	WP_024940062.1	hemolysin III family protein	-
DGN21_RS00240 (DGN21_00255)	67855..68112	+	258	WP_016902922.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DGN21_RS00245 (DGN21_00260)	68130..68399	+	270	WP_228960823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DGN21_RS00250 (DGN21_00265)	68518..69039	-	522	WP_024940060.1	hypothetical protein	-
DGN21_RS00255 (DGN21_00270)	69209..69646	-	438	WP_046345220.1	helix-turn-helix domain-containing protein	-
DGN21_RS00260 (DGN21_00275)	69880..70683	+	804	WP_039518026.1	SDR family NAD(P)-dependent oxidoreductase	-
DGN21_RS00265 (DGN21_00280)	70817..71113	+	297	WP_215825056.1	YciI family protein	-
DGN21_RS00270 (DGN21_00285)	71272..72342	-	1071	WP_215825221.1	ATP-grasp fold amidoligase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	57081..85356	28275

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-80)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10221.75 Da
• Isoelectric Point: 10.1539

>T105366 WP_228960823.1 NZ_CP029263:68130-68399 [Xanthomonas sp. MLO165]
VADLDAIADYIALEDAAAAAALVRRVFAHVEQLAEHPESGSRPQELKRSRYRQIVEPPCRVFYRVDGQRVVVVHVMRSER
LLRKNRLSR

Nucleotide

• Length: 270 bp

>T105366 NZ_CP029263:68130-68399 [Xanthomonas sp. MLO165]
GTGGCCGACCTGGACGCGATTGCCGACTACATTGCGCTTGAGGACGCTGCCGCCGCAGCGGCACTGGTGAGACGGGTGTT
TGCGCATGTCGAGCAATTGGCCGAACACCCGGAGAGTGGAAGCCGGCCGCAGGAGCTCAAGCGCTCCCGCTACCGTCAAA
TCGTCGAGCCACCTTGCCGCGTGTTCTACCGTGTGGACGGGCAACGCGTTGTCGTGGTTCACGTGATGCGCTCGGAGCGG
TTACTTCGGAAGAATCGTCTTTCGCGCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9704.23 Da
• Isoelectric Point: 8.6056

>AT105366 WP_016902922.1 NZ_CP029263:67855-68112 [Xanthomonas sp. MLO165]
MRTELVTTLKRQATELLADVERDKEPILITQHGLPSAYLVDVASYERMQQRMAILEGIARGEMAVAEGRSLSHEQAKQRL
ARWLK

Nucleotide

• Length: 258 bp

>AT105366 NZ_CP029263:67855-68112 [Xanthomonas sp. MLO165]
ATGCGCACAGAACTTGTCACCACGCTCAAGCGGCAGGCCACCGAGCTCCTCGCAGATGTCGAGCGCGACAAGGAGCCGAT
CCTGATCACCCAGCATGGCCTGCCCAGCGCATACCTGGTGGACGTCGCCAGCTATGAACGCATGCAACAGCGGATGGCGA
TCCTCGAAGGAATTGCCCGTGGTGAAATGGCGGTAGCCGAAGGCCGCAGCCTGAGCCACGAACAAGCCAAACAACGCCTG
GCGCGATGGCTGAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A2N7V9L6