Detailed information of TA system TA105359

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 282564..283090
• Replicon: plasmid pOSUEC_D
• Accession: NZ_CP029248
• Organism: Enterobacter hormaechei subsp. xiangfangensis strain OSUVMCKPC4-2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: DF208_RS25990
• Protein ID: WP_000323025.1
• Coordinates: 282564..282851 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: DF208_RS25995
• Protein ID: WP_000534858.1
• Coordinates: 282851..283090 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DF208_RS26705	278134..278310	-	177	WP_001371930.1	hypothetical protein	-
DF208_RS25960	278821..279765	+	945	WP_000778029.1	DUF5417 domain-containing protein	-
DF208_RS25965	279861..280463	+	603	WP_012695474.1	hypothetical protein	-
DF208_RS25970	280523..280873	+	351	WP_000743059.1	hypothetical protein	-
DF208_RS25975	280920..281123	+	204	WP_001015183.1	hypothetical protein	-
DF208_RS25980	281405..281725	+	321	WP_000332796.1	hypothetical protein	-
DF208_RS25985	282334..282492	-	159	WP_001447866.1	type I toxin-antitoxin system Hok family toxin	-
DF208_RS25990	282564..282851	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
DF208_RS25995	282851..283090	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
DF208_RS26005	283353..284276	-	924	WP_000167917.1	cation diffusion facilitator family transporter	-
DF208_RS26010	284476..285048	-	573	WP_001515348.1	cytochrome b/b6 domain-containing protein	-
DF208_RS26015	285524..286762	-	1239	WP_000219087.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ant(3'')-Ia / blaOXA-129 / dfrA21 / aph(6)-Id / aph(3'')-Ib / tet(B) / formA / mcr-9 / blaKPC-4	-	1..354256	354256

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T105359 WP_000323025.1 NZ_CP029248:c282851-282564 [Enterobacter hormaechei subsp. xiangfangensis]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T105359 NZ_CP029248:c282851-282564 [Enterobacter hormaechei subsp. xiangfangensis]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT105359 WP_000534858.1 NZ_CP029248:c283090-282851 [Enterobacter hormaechei subsp. xiangfangensis]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT105359 NZ_CP029248:c283090-282851 [Enterobacter hormaechei subsp. xiangfangensis]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8