Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/FR47-DUF1778
Location 35883..36680 Replicon plasmid pOSUEC_F
Accession NZ_CP029247
Organism Enterobacter hormaechei subsp. xiangfangensis strain OSUVMCKPC4-2

Toxin (Protein)


Gene name KacT Uniprot ID F5S3R9
Locus tag DF208_RS23985 Protein ID WP_006812498.1
Coordinates 36156..36680 (+) Length 175 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5S3R8
Locus tag DF208_RS23980 Protein ID WP_006812497.1
Coordinates 35883..36152 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DF208_RS23955 31301..32641 - 1341 WP_115877092.1 AAA family ATPase -
DF208_RS23960 32702..33427 - 726 WP_115877093.1 hypothetical protein -
DF208_RS23965 33692..34489 + 798 WP_080395882.1 DUF2971 domain-containing protein -
DF208_RS23970 34530..34883 - 354 WP_160859104.1 hypothetical protein -
DF208_RS23975 34889..35554 - 666 WP_023315962.1 AAA family ATPase -
DF208_RS23980 35883..36152 + 270 WP_006812497.1 DUF1778 domain-containing protein Antitoxin
DF208_RS23985 36156..36680 + 525 WP_006812498.1 GNAT family N-acetyltransferase Toxin
DF208_RS26655 36707..36913 - 207 WP_023316023.1 hypothetical protein -
DF208_RS26660 36864..37115 - 252 WP_042863552.1 hypothetical protein -
DF208_RS23995 37117..37809 - 693 WP_006812500.1 membrane protein -
DF208_RS24000 37823..38146 - 324 WP_042863551.1 hypothetical protein -
DF208_RS24005 38221..38316 - 96 Protein_47 receptor-recognizing protein -
DF208_RS24010 38291..38992 - 702 Protein_48 phage tail sheath subtilisin-like domain-containing protein -
DF208_RS24015 39367..39798 - 432 WP_042863549.1 tail fiber assembly protein -
DF208_RS24020 39800..40345 - 546 Protein_50 phage tail protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..108404 108404


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-149)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 175 a.a.        Molecular weight: 19356.13 Da        Isoelectric Point: 8.9844

>T105357 WP_006812498.1 NZ_CP029247:36156-36680 [Enterobacter hormaechei subsp. xiangfangensis]
VANLTIEMFSEEAVYDFSCFDCGETSLNDFLNNRLAQQHSGRILRGYLLLTRDPIPKVMGFYTLSGSCFARNTLPSNTQQ
RKVPYSDAPSVTLGRLAIDKSIQRQGYGETLVAHAMKVVYRASLAVGIYALFVDAKNENAMRFYKQLGFTPLTGDNANSL
FYPTKGIEKLFGGK

Download         Length: 525 bp

>T105357 NZ_CP029247:36156-36680 [Enterobacter hormaechei subsp. xiangfangensis]
GTGGCCAACCTGACCATTGAGATGTTTTCAGAAGAGGCTGTATATGATTTCTCATGCTTCGACTGCGGAGAAACGTCTCT
CAATGATTTTCTTAACAATCGCCTCGCCCAGCAACACAGCGGGCGTATATTACGCGGATATCTGCTTCTGACGAGAGATC
CGATACCGAAAGTGATGGGGTTTTACACGTTGTCAGGGAGCTGCTTTGCAAGAAACACGCTTCCTTCCAACACTCAACAG
CGTAAAGTCCCCTATTCCGACGCCCCAAGCGTTACGCTCGGTCGTCTGGCAATAGACAAAAGTATCCAGCGACAAGGGTA
TGGGGAAACGCTGGTGGCACATGCGATGAAAGTGGTATACCGGGCTTCCCTGGCCGTTGGGATCTACGCTTTGTTTGTTG
ACGCCAAAAATGAGAATGCGATGCGGTTTTATAAGCAGTTAGGCTTTACCCCTCTTACGGGTGATAACGCCAATTCGCTG
TTCTATCCCACAAAAGGCATTGAGAAGTTGTTTGGTGGAAAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10114.51 Da        Isoelectric Point: 4.8483

>AT105357 WP_006812497.1 NZ_CP029247:35883-36152 [Enterobacter hormaechei subsp. xiangfangensis]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNIVMDAISNPPAPNDRLKR
AAERLQSME

Download         Length: 270 bp

>AT105357 NZ_CP029247:35883-36152 [Enterobacter hormaechei subsp. xiangfangensis]
ATGTCAGCACTGAAAAAACAGCGCATCGATCTCAGATTAACCGACGACGACAAGAGCATGATCGAAGAAGCTGCGGCAAT
GACCAACCAGACAATTACTCAGTTTATGGTTGCCAGTGCCTCTGAACGTGCTGCGGAAGTGATAGAGCAACATCGTCGCC
TGATTCTTAGCGAAGAGTCATGGAATATAGTGATGGATGCCATCAGTAACCCGCCTGCACCGAATGATAGGCTGAAACGA
GCTGCCGAGCGTCTACAAAGCATGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0QWY8

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0QUA3

References