Detailed information of TA system TA105344

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 300286..300882
• Replicon: chromosome
• Accession: NZ_CP029246
• Organism: Enterobacter hormaechei subsp. xiangfangensis strain OSUVMCKPC4-2

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A837F9W8
• Locus tag: DF208_RS01470
• Protein ID: WP_023315213.1
• Coordinates: 300580..300882 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: DF208_RS01465
• Protein ID: WP_032610152.1
• Coordinates: 300286..300573 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DF208_RS01460	298658..300289	+	1632	WP_032610151.1	Na/Pi cotransporter family protein	-
DF208_RS01465	300286..300573	-	288	WP_032610152.1	putative addiction module antidote protein	Antitoxin
DF208_RS01470	300580..300882	-	303	WP_023315213.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DF208_RS01475	301080..301952	+	873	WP_003860347.1	23S rRNA pseudouridine(2604) synthase RluF	-
DF208_RS01480	301953..302225	-	273	WP_003860346.1	DUF3811 domain-containing protein	-
DF208_RS01485	302276..303220	-	945	WP_115877045.1	ketopantoate/pantoate/pantothenate transporter PanS	-
DF208_RS01490	303314..304663	-	1350	WP_032608241.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11427.21 Da
• Isoelectric Point: 10.1042

>T105344 WP_023315213.1 NZ_CP029246:c300882-300580 [Enterobacter hormaechei subsp. xiangfangensis]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRVYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T105344 NZ_CP029246:c300882-300580 [Enterobacter hormaechei subsp. xiangfangensis]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10072.60 Da
• Isoelectric Point: 5.7807

>AT105344 WP_032610152.1 NZ_CP029246:c300573-300286 [Enterobacter hormaechei subsp. xiangfangensis]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISLQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Nucleotide

• Length: 288 bp

>AT105344 NZ_CP029246:c300573-300286 [Enterobacter hormaechei subsp. xiangfangensis]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCTGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Structures

Toxin

• AlphaFold DB: A0A837F9W8