Detailed information of TA system TA105341

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 26490..27091
• Replicon: plasmid pTB202
• Accession: NZ_CP029244
• Organism: Escherichia coli strain ECCRA-119

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: C7235_RS26425
• Protein ID: WP_072731915.1
• Coordinates: 26711..27091 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: C7235_RS26420
• Protein ID: WP_072731917.1
• Coordinates: 26490..26711 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7235_RS26410	23522..24754	-	1233	WP_072731919.1	restriction endonuclease subunit S	-
C7235_RS26415	24751..26307	-	1557	WP_001568002.1	type I restriction-modification system subunit M	-
C7235_RS26420	26490..26711	+	222	WP_072731917.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C7235_RS26425	26711..27091	+	381	WP_072731915.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
C7235_RS26430	27096..27275	+	180	WP_000113018.1	hypothetical protein	-
C7235_RS26435	27303..28279	+	977	Protein_22	DUF968 domain-containing protein	-
C7235_RS26440	28295..28992	-	698	Protein_23	IS1 family transposase	-
C7235_RS26450	29184..29303	+	120	WP_001376634.1	ash family protein	-
C7235_RS26455	29322..29543	+	222	WP_072019028.1	host cell division inhibitor Icd-like protein	-
C7235_RS26460	29540..30655	+	1116	WP_033551397.1	phage antirepressor KilAC domain-containing protein	-
C7235_RS26465	30688..31539	-	852	WP_000611664.1	hypothetical protein	-
C7235_RS26470	31650..31859	-	210	WP_000874156.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(A) / dfrA12 / aadA2 / qacE / sul1 / blaTEM-1B / mph(A)	-	1..139629	139629
	-	flank	IS/Tn	-	-	32047..33027	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13631.32 Da
• Isoelectric Point: 5.1514

>T105341 WP_072731915.1 NZ_CP029244:26711-27091 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSSE

Nucleotide

• Length: 381 bp

>T105341 NZ_CP029244:26711-27091 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGCATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACTTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGTGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTTCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8145.15 Da
• Isoelectric Point: 4.7708

>AT105341 WP_072731917.1 NZ_CP029244:26490-26711 [Escherichia coli]
MQSINFRIARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT105341 NZ_CP029244:26490-26711 [Escherichia coli]
ATGCAATCCATTAACTTCCGTATCGCGCGGGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA