Detailed information of TA system TA105329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2509935..2510573
• Replicon: chromosome
• Accession: NZ_CP029242
• Organism: Escherichia coli strain ECCRA-119

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: C7235_RS13040
• Protein ID: WP_000813794.1
• Coordinates: 2510397..2510573 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C7235_RS13035
• Protein ID: WP_001270285.1
• Coordinates: 2509935..2510351 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7235_RS13015	2505087..2506028	-	942	WP_001251313.1	ABC transporter permease	-
C7235_RS13020	2506029..2507042	-	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
C7235_RS13025	2507060..2508205	-	1146	WP_000034363.1	ABC transporter substrate-binding protein	-
C7235_RS13030	2508450..2509856	-	1407	WP_000760590.1	PLP-dependent aminotransferase family protein	-
C7235_RS13035	2509935..2510351	-	417	WP_001270285.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
C7235_RS13040	2510397..2510573	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
C7235_RS13045	2510795..2511025	+	231	WP_000494244.1	YncJ family protein	-
C7235_RS13050	2511117..2513078	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
C7235_RS13055	2513151..2513687	-	537	WP_000429153.1	DNA-binding transcriptional regulator SutR	-
C7235_RS13060	2513740..2514954	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2514994..2516142	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T105329 WP_000813794.1 NZ_CP029242:c2510573-2510397 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T105329 NZ_CP029242:c2510573-2510397 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15247.61 Da
• Isoelectric Point: 4.6115

>AT105329 WP_001270285.1 NZ_CP029242:c2510351-2509935 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT105329 NZ_CP029242:c2510351-2509935 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTGACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB