Detailed information of TA system TA105315

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 841504..842336
• Replicon: chromosome
• Accession: NZ_CP029242
• Organism: Escherichia coli strain ECCRA-119

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: C7235_RS04510
• Protein ID: WP_000854753.1
• Coordinates: 841504..841878 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: C7235_RS04515
• Protein ID: WP_001278232.1
• Coordinates: 841968..842336 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7235_RS04475	836568..837167	+	600	WP_001255040.1	type II secretion system minor pseudopilin GspJ	-
C7235_RS04480	837170..838147	+	978	WP_000633222.1	type II secretion system minor pseudopilin GspK	-
C7235_RS04485	838144..839322	+	1179	WP_000094974.1	type II secretion system protein GspL	-
C7235_RS04490	839324..839860	+	537	WP_000942786.1	GspM family type II secretion system protein YghD	-
C7235_RS04495	840141..840713	-	573	WP_001290246.1	DUF4942 domain-containing protein	-
C7235_RS04500	840810..841007	-	198	WP_000839281.1	DUF957 domain-containing protein	-
C7235_RS04505	841019..841507	-	489	WP_000777545.1	hypothetical protein	-
C7235_RS04510	841504..841878	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
C7235_RS04515	841968..842336	-	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
C7235_RS04520	842499..842720	-	222	WP_000692329.1	DUF987 domain-containing protein	-
C7235_RS04525	842783..843259	-	477	WP_001186726.1	RadC family protein	-
C7235_RS04530	843275..843760	-	486	WP_000849582.1	antirestriction protein	-
C7235_RS04535	843815..844633	-	819	WP_001234620.1	DUF945 domain-containing protein	-
C7235_RS04545	844733..844966	-	234	WP_001119729.1	DUF905 family protein	-
C7235_RS04550	845045..845500	-	456	WP_072645706.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T105315 WP_000854753.1 NZ_CP029242:c841878-841504 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T105315 NZ_CP029242:c841878-841504 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT105315 WP_001278232.1 NZ_CP029242:c842336-841968 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT105315 NZ_CP029242:c842336-841968 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTACTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: E3PJ72