Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 330992..331623 Replicon chromosome
Accession NZ_CP029242
Organism Escherichia coli strain ECCRA-119

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag C7235_RS01750 Protein ID WP_001259388.1
Coordinates 330992..331267 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag C7235_RS01755 Protein ID WP_000593555.1
Coordinates 331264..331623 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7235_RS01735 325996..327063 + 1068 WP_000361482.1 HlyD family efflux transporter periplasmic adaptor subunit -
C7235_RS01740 327060..329795 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
C7235_RS01745 329795..330919 + 1125 WP_001615305.1 ABC transporter permease -
C7235_RS01750 330992..331267 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
C7235_RS01755 331264..331623 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C7235_RS01760 331743..332144 - 402 WP_001190064.1 nickel-responsive transcriptional regulator NikR -
C7235_RS01765 332150..332956 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
C7235_RS01770 332953..333717 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
C7235_RS01775 333717..334550 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
C7235_RS01780 334547..335491 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T105313 WP_001259388.1 NZ_CP029242:330992-331267 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T105313 NZ_CP029242:330992-331267 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT105313 WP_000593555.1 NZ_CP029242:331264-331623 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT105313 NZ_CP029242:331264-331623 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References