Detailed information of TA system TA105279

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 264665..265344
• Replicon: chromosome
• Accession: NZ_CP029239
• Organism: Escherichia coli strain BH212

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: DF195_RS01260
• Protein ID: WP_000854672.1
• Coordinates: 264665..265006 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: DF195_RS01265
• Protein ID: WP_000070395.1
• Coordinates: 265027..265344 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DF195_RS01235	259942..260343	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
DF195_RS01240	260382..261437	-	1056	WP_000749863.1	phosphoporin PhoE	-
DF195_RS01245	261725..262828	+	1104	WP_001285288.1	glutamate 5-kinase	-
DF195_RS01250	262840..264093	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
DF195_RS01260	264665..265006	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
DF195_RS01265	265027..265344	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
DF195_RS01270	265363..265584	-	222	WP_000691994.1	DUF987 domain-containing protein	-
DF195_RS01275	265593..266069	-	477	WP_000811693.1	RadC family protein	-
DF195_RS01280	266085..266543	-	459	WP_000211838.1	antirestriction protein	-
DF195_RS01285	266641..266880	-	240	WP_000194654.1	DUF905 domain-containing protein	-
DF195_RS01290	266957..267424	-	468	WP_001547765.1	hypothetical protein	-
DF195_RS01295	267447..267890	-	444	WP_000824223.1	hypothetical protein	-
DF195_RS01300	267890..268117	-	228	WP_001548158.1	hypothetical protein	-
DF195_RS01305	268113..268304	-	192	Protein_253	DeoR family transcriptional regulator	-
DF195_RS01310	268521..269342	-	822	WP_000197389.1	DUF945 domain-containing protein	-
DF195_RS01315	269434..270297	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T105279 WP_000854672.1 NZ_CP029239:c265006-264665 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T105279 NZ_CP029239:c265006-264665 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT105279 WP_000070395.1 NZ_CP029239:c265344-265027 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT105279 NZ_CP029239:c265344-265027 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684