Detailed information of TA system TA105259

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2077112..2077943
• Replicon: chromosome
• Accession: NZ_CP029238
• Organism: Escherichia coli strain CJ236

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: DF192_RS10355
• Protein ID: WP_000854814.1
• Coordinates: 2077569..2077943 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: DF192_RS10350
• Protein ID: WP_001285584.1
• Coordinates: 2077112..2077480 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DF192_RS10335	2074779..2076311	+	1533	WP_001350525.1	hypothetical protein	-
DF192_RS10340	2076308..2076754	+	447	WP_000187523.1	RadC family protein	-
DF192_RS10345	2076817..2077038	+	222	WP_000692323.1	DUF987 domain-containing protein	-
DF192_RS10350	2077112..2077480	+	369	WP_001285584.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
DF192_RS10355	2077569..2077943	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
DF192_RS10360	2077940..2078134	+	195	WP_000988600.1	hypothetical protein	-
DF192_RS10365	2078147..2078260	+	114	WP_001161660.1	DUF957 domain-containing protein	-
DF192_RS10370	2078549..2078677	-	129	Protein_2014	transposase domain-containing protein	-
DF192_RS10375	2078749..2078931	+	183	WP_001016348.1	hypothetical protein	-
DF192_RS10380	2079032..2079361	-	330	WP_000450409.1	DUF496 family protein	-
DF192_RS10385	2079533..2080591	-	1059	WP_001200891.1	FUSC family protein	-
DF192_RS10390	2080789..2081262	-	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
DF192_RS10395	2081381..2082547	-	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T105259 WP_000854814.1 NZ_CP029238:2077569-2077943 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T105259 NZ_CP029238:2077569-2077943 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT105259 WP_001285584.1 NZ_CP029238:2077112-2077480 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT105259 NZ_CP029238:2077112-2077480 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• PDB: 2H28
• AlphaFold DB: A0A1M2E8G6