Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 123441..124243 Replicon plasmid pSF45436a
Accession NZ_CP029233
Organism Sinorhizobium fredii CCBAU 45436

Toxin (Protein)


Gene name TacT3 Uniprot ID I3XHG7
Locus tag AB395_RS30345 Protein ID WP_014857792.1
Coordinates 123716..124243 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name TacA3 Uniprot ID A0A178YHY5
Locus tag AB395_RS30340 Protein ID WP_015633362.1
Coordinates 123441..123719 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AB395_RS30315 119756..120942 - 1187 Protein_115 IS3 family transposase -
AB395_RS30320 120940..121137 + 198 Protein_116 phasin family protein -
AB395_RS30325 121364..122076 + 713 Protein_117 IS6 family transposase -
AB395_RS34220 122060..122410 + 351 WP_100209425.1 hypothetical protein -
AB395_RS30335 122490..123347 + 858 WP_014857794.1 hypothetical protein -
AB395_RS30340 123441..123719 + 279 WP_015633362.1 DUF1778 domain-containing protein Antitoxin
AB395_RS30345 123716..124243 + 528 WP_014857792.1 hypothetical protein Toxin
AB395_RS30350 124367..126214 - 1848 WP_037403046.1 AAA family ATPase -
AB395_RS30355 126214..126702 - 489 WP_015633363.1 hypothetical protein -
AB395_RS30360 126690..127721 - 1032 WP_014857789.1 hypothetical protein -
AB395_RS30365 127800..128879 + 1080 WP_037446536.1 IS630 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - gmd 1..418014 418014


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Vibrio phage ICP1, Orf50

(5-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 18735.62 Da        Isoelectric Point: 8.2948

>T105233 WP_014857792.1 NZ_CP029233:123716-124243 [Sinorhizobium fredii CCBAU 45436]
MTLPAWHEEPIAKSHGRAVFDCGDGQMNEFLLRFARQSHEQNASKTFCAIDDAVPGRILGFYTIAPSAVEHATVPDAMTR
GLSRHDVPGFKLARLATDRAVAGQGLGGRLLAAAALRCLRLAGEGGGLLLIIDAKGERAANWYASYGAEPLKDKPLTLVM
PLITFAADLRARGLL

Download         Length: 528 bp

>T105233 NZ_CP029233:123716-124243 [Sinorhizobium fredii CCBAU 45436]
ATGACTTTACCTGCATGGCATGAGGAACCGATCGCTAAGTCTCACGGTCGGGCAGTTTTCGATTGCGGCGACGGTCAGAT
GAACGAGTTTTTGCTCCGCTTTGCTCGACAGAGCCATGAGCAGAATGCCTCGAAAACCTTCTGTGCTATCGACGACGCAG
TGCCCGGCCGTATTCTTGGGTTCTATACGATCGCGCCCTCGGCAGTCGAGCATGCGACTGTGCCAGATGCAATGACACGC
GGTCTTTCCCGCCATGACGTGCCAGGCTTCAAGCTCGCGCGATTGGCTACCGACCGCGCGGTTGCCGGGCAGGGACTGGG
AGGCCGGCTCCTAGCCGCCGCCGCGCTCCGCTGCCTGCGACTTGCAGGAGAGGGCGGGGGACTTTTACTGATCATCGATG
CCAAAGGCGAGCGAGCCGCCAACTGGTACGCTTCCTATGGTGCCGAACCGCTAAAGGACAAGCCGCTAACTCTCGTAATG
CCGCTCATCACCTTCGCGGCCGACTTGCGTGCTAGAGGTCTCCTTTGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 9999.57 Da        Isoelectric Point: 10.0863

>AT105233 WP_015633362.1 NZ_CP029233:123441-123719 [Sinorhizobium fredii CCBAU 45436]
MPSIANENSERMSLRVPATAKAKLVRAAALRNTDLTDFVTQSALREAEAVIEEAETIKVSKRDHLRILELLENPPKPNAK
LRAAIAGLPGSL

Download         Length: 279 bp

>AT105233 NZ_CP029233:123441-123719 [Sinorhizobium fredii CCBAU 45436]
ATGCCGAGCATAGCGAACGAGAACAGCGAACGTATGAGTTTGAGGGTGCCTGCCACGGCCAAGGCTAAACTCGTACGCGC
TGCGGCACTTCGTAATACCGATCTGACGGATTTCGTGACGCAATCCGCGCTGCGAGAAGCAGAGGCTGTGATCGAGGAAG
CCGAGACGATCAAAGTGTCGAAGCGCGATCATCTTCGCATTCTTGAGCTTCTGGAAAATCCGCCTAAACCTAACGCCAAG
CTGCGCGCGGCGATTGCCGGTTTGCCTGGCAGCCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I3XHG7

Antitoxin

Source ID Structure
AlphaFold DB A0A178YHY5

References