Detailed information of TA system TA105231

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1197597..1198258
• Replicon: plasmid pSF45436b
• Accession: NZ_CP029232
• Organism: Sinorhizobium fredii CCBAU 45436

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A2U8GCA8
• Locus tag: AB395_RS25900
• Protein ID: WP_037452921.1
• Coordinates: 1197821..1198258 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2U8GCN0
• Locus tag: AB395_RS25895
• Protein ID: WP_037460318.1
• Coordinates: 1197597..1197824 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB395_RS34470	1192632..1192844	+	213	WP_193378139.1	hypothetical protein	-
AB395_RS25875	1192890..1193672	-	783	WP_109023413.1	anti-sigma factor	-
AB395_RS25880	1193669..1194211	-	543	WP_037400245.1	RNA polymerase sigma factor	-
AB395_RS25885	1194354..1195757	+	1404	WP_037452938.1	S8 family serine peptidase	-
AB395_RS25890	1196200..1197156	-	957	WP_014332035.1	patatin-like phospholipase family protein	-
AB395_RS25895	1197597..1197824	+	228	WP_037460318.1	hypothetical protein	Antitoxin
AB395_RS25900	1197821..1198258	+	438	WP_037452921.1	PIN domain-containing protein	Toxin
AB395_RS25905	1198298..1198831	-	534	WP_014332038.1	Rrf2 family transcriptional regulator	-
AB395_RS25910	1199060..1199476	+	417	WP_037437355.1	cupin domain-containing protein	-
AB395_RS25915	1199501..1199956	+	456	WP_037452917.1	carboxymuconolactone decarboxylase family protein	-
AB395_RS25920	1200072..1200323	+	252	Protein_1092	peptide-methionine (R)-S-oxide reductase MsrB	-
AB395_RS25925	1200324..1200830	+	507	WP_037452914.1	peptide-methionine (S)-S-oxide reductase MsrA	-
AB395_RS25930	1201057..1202307	+	1251	WP_037452910.1	bifunctional alpha/beta hydrolase/OsmC family protein	-
AB395_RS25935	1202475..1202900	-	426	WP_037452906.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pseB / katA	1..1961287	1961287

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-132)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15263.49 Da
• Isoelectric Point: 6.7480

>T105231 WP_037452921.1 NZ_CP029232:1197821-1198258 [Sinorhizobium fredii CCBAU 45436]
VTFLLDVNVLIALIDPGHVAHDDAHDWFASTGKTAWATCPITENGVLRIVGNPKYPNSPGSPALVMEIVGKLRSLPGHCF
WPDDVSLVGSGDIAPGKILTSGQVTDTYLLALAKAHDGQLATFDRKLTAAAVTRGNSALHLITGK

Nucleotide

• Length: 438 bp

>T105231 NZ_CP029232:1197821-1198258 [Sinorhizobium fredii CCBAU 45436]
GTGACCTTCCTGCTCGACGTCAATGTGCTGATTGCCTTGATCGATCCGGGCCATGTAGCCCATGATGATGCGCACGATTG
GTTCGCATCGACCGGCAAGACCGCGTGGGCGACCTGTCCCATTACCGAAAACGGTGTCCTCCGCATTGTCGGCAATCCCA
AATACCCCAATTCTCCCGGCTCACCGGCACTCGTGATGGAGATCGTTGGCAAGTTGCGGTCGCTCCCTGGTCATTGTTTT
TGGCCGGACGACGTGAGCCTCGTCGGGTCAGGTGATATCGCTCCCGGGAAAATCCTGACTTCGGGGCAGGTAACCGATAC
CTATCTGCTTGCGCTCGCGAAGGCGCATGACGGCCAGTTGGCGACATTCGATCGCAAGCTAACAGCAGCGGCGGTCACGA
GGGGCAACTCTGCCTTGCACCTGATCACCGGCAAATAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8141.32 Da
• Isoelectric Point: 7.0110

>AT105231 WP_037460318.1 NZ_CP029232:1197597-1197824 [Sinorhizobium fredii CCBAU 45436]
MRTTLAIDDDVLIAAKAMAAQQHRSLGDVISDLARRSLRRPRSGGERNGIPLLSTQPDAPPVTLEIVNALRDELP

Nucleotide

• Length: 228 bp

>AT105231 NZ_CP029232:1197597-1197824 [Sinorhizobium fredii CCBAU 45436]
ATGAGAACGACACTTGCGATCGATGACGATGTCCTGATCGCCGCCAAGGCGATGGCAGCCCAGCAGCACCGCAGTCTCGG
CGATGTCATTTCCGATCTGGCGAGGCGTTCTTTGCGCCGGCCGCGCAGTGGTGGCGAGCGCAATGGAATTCCTCTTCTGT
CGACGCAACCCGATGCCCCGCCAGTTACCCTCGAAATCGTCAACGCCTTGCGTGACGAACTGCCGTGA

Structures

Toxin

• AlphaFold DB: A0A2U8GCA8

Antitoxin

• AlphaFold DB: A0A2U8GCN0