Detailed information of TA system TA105230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 425469..426103
• Replicon: plasmid pSF45436b
• Accession: NZ_CP029232
• Organism: Sinorhizobium fredii CCBAU 45436

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: AB395_RS22315
• Protein ID: WP_037454554.1
• Coordinates: 425915..426103 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: G9AFA0
• Locus tag: AB395_RS22310
• Protein ID: WP_014331390.1
• Coordinates: 425469..425909 (-)
• Length: 147 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB395_RS22295	421391..422002	+	612	WP_037454563.1	SCO family protein	-
AB395_RS22300	422084..423337	+	1254	WP_037454583.1	5-aminolevulinate synthase	-
AB395_RS22305	423414..425054	+	1641	WP_037454560.1	chaperonin GroEL	-
AB395_RS22310	425469..425909	-	441	WP_014331390.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AB395_RS22315	425915..426103	-	189	WP_037454554.1	type II toxin-antitoxin system HicA family toxin	Toxin
AB395_RS22320	426544..429666	+	3123	WP_037454551.1	PAS domain S-box protein	-
AB395_RS22325	429795..430187	-	393	WP_014331393.1	response regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pseB / katA	1..1961287	1961287

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6915.12 Da
• Isoelectric Point: 10.9086

>T105230 WP_037454554.1 NZ_CP029232:c426103-425915 [Sinorhizobium fredii CCBAU 45436]
MKSGDIIVALQKDGWYQVATKGSHVQFKHPEKPGRVTVPHPKRDIPIGTLKSIEKQAGLKLR

Nucleotide

• Length: 189 bp

>T105230 NZ_CP029232:c426103-425915 [Sinorhizobium fredii CCBAU 45436]
ATGAAGAGCGGTGATATCATTGTGGCCTTGCAAAAGGATGGCTGGTACCAGGTCGCCACCAAAGGCAGCCACGTGCAGTT
TAAGCATCCCGAAAAACCCGGGCGCGTCACTGTTCCACACCCGAAAAGGGACATCCCGATCGGAACACTGAAGAGTATCG
AGAAACAAGCCGGACTTAAACTGAGGTAA

Antitoxin

Protein

• Length: 147 a.a.
• Molecular weight: 15639.37 Da
• Isoelectric Point: 4.1962

>AT105230 WP_014331390.1 NZ_CP029232:c425909-425469 [Sinorhizobium fredii CCBAU 45436]
MRNYIGLIHKDTDSDYGVSFPDFPGLITAGTDLDDARAMAEEALALHIEGLAEDGEAIPEPSTLEAVMADSENRDGVAIL
VGVKTEAKRAVRVNITLPEDVLKAIDAFAEAQGLTRSGFLVRAAKHEIGHANDDYNDNYAEARLGA

Nucleotide

• Length: 441 bp

>AT105230 NZ_CP029232:c425909-425469 [Sinorhizobium fredii CCBAU 45436]
ATGCGCAATTATATCGGATTGATCCATAAGGACACCGACAGTGATTACGGTGTGTCCTTTCCCGATTTTCCTGGTCTGAT
CACTGCTGGAACTGACCTCGACGATGCGCGAGCTATGGCCGAAGAAGCCTTGGCCCTACACATCGAAGGTCTAGCCGAAG
ACGGCGAAGCGATTCCCGAACCTTCCACACTGGAGGCGGTGATGGCAGACTCTGAGAACCGAGACGGCGTTGCAATTCTA
GTTGGCGTCAAGACTGAAGCCAAACGAGCGGTTCGCGTAAATATCACCCTGCCAGAAGACGTCCTAAAGGCAATCGATGC
GTTCGCCGAAGCCCAAGGACTGACCCGATCAGGCTTCTTGGTTCGGGCTGCCAAGCATGAGATCGGCCATGCAAACGACG
ACTACAACGATAACTACGCAGAGGCGCGGCTAGGGGCCTAA

Structures

Antitoxin

• AlphaFold DB: G9AFA0