Detailed information of TA system TA105229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 139741..140273
• Replicon: plasmid pSF45436b
• Accession: NZ_CP029232
• Organism: Sinorhizobium fredii CCBAU 45436

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G9AEI1
• Locus tag: AB395_RS20955
• Protein ID: WP_014331123.1
• Coordinates: 139950..140273 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G9AEI0
• Locus tag: AB395_RS20950
• Protein ID: WP_014331122.1
• Coordinates: 139741..139953 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB395_RS20925	135402..136421	-	1020	WP_014331118.1	LacI family DNA-binding transcriptional regulator	-
AB395_RS20930	136704..137507	+	804	WP_014331119.1	transporter substrate-binding domain-containing protein	-
AB395_RS20935	137575..138278	+	704	Protein_125	amino acid ABC transporter permease	-
AB395_RS20940	138275..138934	+	660	WP_037455121.1	amino acid ABC transporter permease	-
AB395_RS20945	138915..139646	+	732	WP_037455118.1	amino acid ABC transporter ATP-binding protein	-
AB395_RS20950	139741..139953	+	213	WP_014331122.1	antitoxin MazE family protein	Antitoxin
AB395_RS20955	139950..140273	+	324	WP_014331123.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AB395_RS20960	140320..141336	-	1017	WP_037455185.1	LacI family DNA-binding transcriptional regulator	-
AB395_RS20965	141497..141928	-	432	WP_014331125.1	helix-turn-helix transcriptional regulator	-
AB395_RS20970	142018..142833	+	816	WP_014331126.1	oxidoreductase	-
AB395_RS20975	142892..143893	+	1002	WP_014331127.1	NADP-dependent oxidoreductase	-
AB395_RS20980	144044..144376	-	333	WP_014331128.1	hypothetical protein	-
AB395_RS20985	144373..145260	-	888	WP_014331129.1	NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pseB / katA	1..1961287	1961287

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• Antitoxin MazE-like (6-61)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11945.80 Da
• Isoelectric Point: 7.0140

>T105229 WP_014331123.1 NZ_CP029232:139950-140273 [Sinorhizobium fredii CCBAU 45436]
MRRGDIVTVALQGDNGKPRPAVIVQADAFDRIERLFIIPLTSDIAETPYYRVTIEPSENSGLRARSQVMLDRMTNTPRSK
IGSVIGHIDRETMQEIDRLLAIILGIA

Nucleotide

• Length: 324 bp

>T105229 NZ_CP029232:139950-140273 [Sinorhizobium fredii CCBAU 45436]
ATGAGGCGCGGCGATATCGTGACCGTCGCGCTTCAGGGCGATAATGGCAAACCACGTCCTGCGGTGATCGTGCAAGCCGA
TGCCTTCGACAGGATCGAGCGCCTTTTCATCATTCCACTGACCAGCGATATTGCCGAGACGCCCTATTACCGGGTCACCA
TCGAGCCGAGCGAAAATTCCGGCTTGCGCGCAAGATCACAGGTCATGCTCGACCGCATGACGAATACTCCACGAAGCAAG
ATTGGCAGCGTCATCGGTCATATTGATCGCGAAACGATGCAGGAAATCGACCGCCTTCTGGCGATCATCCTTGGCATTGC
ATAA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 8047.89 Da
• Isoelectric Point: 4.7571

>AT105229 WP_014331122.1 NZ_CP029232:139741-139953 [Sinorhizobium fredii CCBAU 45436]
MSSATRQQRYREKMRAAGFKPVTIWVPDTSNPTFAKEAARQAEILNAKPRSDNDDFWDAVAEDAFRDIEE

Nucleotide

• Length: 213 bp

>AT105229 NZ_CP029232:139741-139953 [Sinorhizobium fredii CCBAU 45436]
ATGAGCAGTGCAACCCGCCAACAGCGCTATCGCGAAAAGATGCGCGCCGCCGGCTTCAAGCCGGTAACGATCTGGGTGCC
AGACACCAGCAATCCGACGTTCGCCAAGGAGGCCGCACGCCAGGCTGAAATCCTGAATGCCAAGCCGCGATCCGACAACG
ATGATTTTTGGGATGCGGTCGCCGAAGATGCGTTCAGGGATATTGAGGAATGA

Structures

Toxin

• AlphaFold DB: G9AEI1

Antitoxin

• AlphaFold DB: G9AEI0