Detailed information of TA system TA105228

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 131232..131880
• Replicon: chromosome
• Accession: NZ_CP029231
• Organism: Sinorhizobium fredii CCBAU 45436

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A2U8FXN3
• Locus tag: AB395_RS00625
• Protein ID: WP_037450644.1
• Coordinates: 131626..131880 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: AB395_RS00620
• Protein ID: WP_037391843.1
• Coordinates: 131232..131612 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB395_RS00605	128948..129613	+	666	WP_037450648.1	CDP-alcohol phosphatidyltransferase family protein	-
AB395_RS00610	129610..130221	+	612	WP_037450646.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
AB395_RS00615	130229..131170	+	942	WP_014327148.1	phosphatidate cytidylyltransferase	-
AB395_RS00620	131232..131612	-	381	WP_037391843.1	helix-turn-helix transcriptional regulator	Antitoxin
AB395_RS00625	131626..131880	-	255	WP_037450644.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AB395_RS00635	132258..133268	-	1011	WP_037450642.1	nitronate monooxygenase family protein	-
AB395_RS00640	133553..134725	+	1173	WP_037450639.1	hypothetical protein	-
AB395_RS00645	134741..135643	-	903	WP_014327194.1	polyphosphate kinase 2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-79)

Antitoxin

• Cro/C1-type helix-turn-helix domain (75-125)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9433.98 Da
• Isoelectric Point: 10.7217

>T105228 WP_037450644.1 NZ_CP029231:c131880-131626 [Sinorhizobium fredii CCBAU 45436]
MKTIRYTREALKTLTQIPANVAKRIRSKIEQYASDPASLANNIKALKGLQGTYRLRVGDWRVIFTEDGEIIAIKKIAPRG
GAYD

Nucleotide

• Length: 255 bp

>T105228 NZ_CP029231:c131880-131626 [Sinorhizobium fredii CCBAU 45436]
ATGAAAACGATCCGGTACACCCGTGAAGCGCTGAAAACCTTGACACAAATTCCCGCGAACGTTGCCAAGCGGATCCGCTC
GAAAATCGAGCAATATGCATCGGACCCGGCAAGTCTCGCTAACAACATCAAGGCACTGAAAGGGTTGCAAGGTACATATC
GGCTTCGGGTCGGAGACTGGCGCGTTATCTTTACGGAAGACGGCGAAATCATCGCCATCAAGAAGATCGCCCCGCGCGGC
GGCGCATACGACTAG

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 14006.05 Da
• Isoelectric Point: 4.4452

>AT105228 WP_037391843.1 NZ_CP029231:c131612-131232 [Sinorhizobium fredii CCBAU 45436]
MVLQFITTPAGDRMVVMAEDEYKMLLHAAEAAEDAEDVADVKRINERLARGEEELIPADIVNRILDGESKVKVWREYRKL
TLRELAAKVDISPAYLSQIEGGSRDGTFETMKKIAAALGITVDELA

Nucleotide

• Length: 381 bp

>AT105228 NZ_CP029231:c131612-131232 [Sinorhizobium fredii CCBAU 45436]
ATGGTACTACAGTTCATCACTACCCCGGCCGGCGACCGAATGGTCGTCATGGCCGAGGACGAATACAAGATGCTTTTGCA
TGCCGCAGAGGCTGCCGAGGACGCCGAAGACGTCGCGGACGTAAAGCGGATCAATGAGCGCCTAGCTCGCGGCGAAGAAG
AGTTGATCCCCGCCGATATCGTCAACCGCATCCTTGATGGCGAAAGCAAGGTGAAGGTCTGGCGCGAGTACAGGAAACTA
ACCCTCCGAGAACTCGCGGCCAAGGTCGATATCAGCCCGGCGTACCTTTCTCAAATCGAGGGCGGCAGCCGCGACGGCAC
CTTTGAGACGATGAAGAAGATAGCGGCCGCTCTCGGTATCACGGTGGACGAACTCGCATAA

Structures

Toxin

• AlphaFold DB: A0A2U8FXN3