Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 739206..739835 Replicon chromosome
Accession NZ_CP029206
Organism Actinobacillus porcitonsillarum strain 9953L55

Toxin (Protein)


Gene name hicA Uniprot ID A0A2U8FJZ3
Locus tag DDU33_RS03775 Protein ID WP_005820924.1
Coordinates 739206..739388 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2U8FI87
Locus tag DDU33_RS03780 Protein ID WP_005820921.1
Coordinates 739431..739835 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DDU33_RS03755 735061..736239 - 1179 WP_108923248.1 pentaheme c-type cytochrome TorC -
DDU33_RS03760 736590..737660 - 1071 WP_108923250.1 MFS transporter -
DDU33_RS03765 737728..738192 + 465 WP_005820928.1 nuclear transport factor 2 family protein -
DDU33_RS03770 738200..739117 + 918 WP_108923252.1 N-acetylmuramic acid 6-phosphate etherase -
DDU33_RS03775 739206..739388 + 183 WP_005820924.1 type II toxin-antitoxin system HicA family toxin Toxin
DDU33_RS03780 739431..739835 + 405 WP_005820921.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
DDU33_RS03785 740167..740457 + 291 WP_108923254.1 hypothetical protein -
DDU33_RS03790 740670..741359 + 690 WP_108923256.1 pirin family protein -
DDU33_RS03795 741621..742355 + 735 WP_108923258.1 phosphoadenylyl-sulfate reductase -
DDU33_RS03800 742383..743309 + 927 WP_108923260.1 sulfate adenylyltransferase subunit CysD -
DDU33_RS03805 743534..744826 + 1293 WP_108923262.1 50S ribosome-binding GTPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6879.06 Da        Isoelectric Point: 10.5939

>T105220 WP_005820924.1 NZ_CP029206:739206-739388 [Actinobacillus porcitonsillarum]
MDSRTLKKMLEDDGWYVVDVKGSHHQFKHNEKKGRVTLPHPKKDLPIKTVKSILKQAGLA

Download         Length: 183 bp

>T105220 NZ_CP029206:739206-739388 [Actinobacillus porcitonsillarum]
ATGGATAGTCGAACGTTAAAGAAAATGCTAGAAGATGATGGCTGGTATGTTGTAGATGTAAAAGGTAGTCATCACCAATT
CAAGCATAATGAAAAGAAAGGGAGAGTAACACTCCCGCATCCAAAAAAAGACTTACCAATAAAAACTGTTAAATCTATAT
TAAAACAGGCTGGGTTAGCATAG

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14747.75 Da        Isoelectric Point: 4.3898

>AT105220 WP_005820921.1 NZ_CP029206:739431-739835 [Actinobacillus porcitonsillarum]
MLYPIAIEQGDEQQAYGVIVPDIPGCFSAGDTLEEAFQNAKEAIEFHLEGLVEDGEEIPKAKSLEVHKNNPELSGLTLAL
VEVDITHLLGKSEKINVTLPSLLIRRIDEFVASHPNYKTRSSFLAQVASEKIFA

Download         Length: 405 bp

>AT105220 NZ_CP029206:739431-739835 [Actinobacillus porcitonsillarum]
ATGCTTTATCCAATTGCAATCGAACAAGGTGATGAACAACAGGCTTATGGTGTTATCGTACCAGATATTCCAGGTTGTTT
TTCTGCTGGTGATACATTAGAAGAGGCATTTCAAAATGCCAAAGAAGCTATTGAGTTCCATTTAGAAGGATTGGTCGAAG
ATGGTGAAGAAATTCCCAAGGCGAAATCATTAGAAGTACATAAAAATAATCCAGAGCTTTCGGGATTAACCTTAGCACTG
GTTGAAGTTGATATTACTCATTTATTAGGTAAGTCAGAAAAAATCAATGTAACTTTGCCTTCGTTACTTATTCGCCGTAT
TGATGAATTTGTTGCAAGTCACCCAAATTATAAAACTCGTAGCAGTTTTTTAGCACAAGTGGCAAGCGAGAAAATTTTTG
CGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U8FJZ3

Antitoxin

Source ID Structure
AlphaFold DB A0A2U8FI87

References