Detailed information of TA system TA105191

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 4622556..4623187
• Replicon: chromosome
• Accession: NZ_CP029190
• Organism: Streptomyces venezuelae strain ATCC 21782

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DEJ50_RS20710
• Protein ID: WP_150209450.1
• Coordinates: 4622556..4622975 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: DEJ50_RS20715
• Protein ID: WP_150209451.1
• Coordinates: 4622972..4623187 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DEJ50_RS20675	4618603..4619475	-	873	WP_150209443.1	hypothetical protein	-
DEJ50_RS20680	4619497..4620030	-	534	WP_150209444.1	SigE family RNA polymerase sigma factor	-
DEJ50_RS20685	4620171..4620674	+	504	WP_150209445.1	GNAT family N-acetyltransferase	-
DEJ50_RS20690	4620653..4621138	+	486	WP_190344995.1	macro domain-containing protein	-
DEJ50_RS20695	4621123..4621368	-	246	WP_150209447.1	DUF397 domain-containing protein	-
DEJ50_RS20700	4621365..4622159	-	795	WP_150209448.1	helix-turn-helix transcriptional regulator	-
DEJ50_RS20705	4622249..4622497	-	249	WP_150209449.1	hypothetical protein	-
DEJ50_RS20710	4622556..4622975	-	420	WP_150209450.1	PIN domain nuclease	Toxin
DEJ50_RS20715	4622972..4623187	-	216	WP_150209451.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
DEJ50_RS20720	4623248..4623685	-	438	WP_150212254.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
DEJ50_RS20725	4623785..4624465	-	681	WP_150209452.1	alpha/beta fold hydrolase	-
DEJ50_RS20730	4624479..4625429	-	951	WP_150212255.1	NADP-dependent oxidoreductase	-
DEJ50_RS20735	4625549..4626532	+	984	WP_150209453.1	helix-turn-helix domain-containing protein	-
DEJ50_RS20740	4626598..4627512	+	915	WP_150209454.1	LysR family transcriptional regulator	-
DEJ50_RS20745	4627618..4627812	-	195	WP_150209455.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-119)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-43)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15593.74 Da
• Isoelectric Point: 4.9616

>T105191 WP_150209450.1 NZ_CP029190:c4622975-4622556 [Streptomyces venezuelae]
MNTQRYLIDKSALARYPRPAVRAVLDPLHRAGLLAVCGAVELEILYSARSKADAERIRDEMRGFEWLPTPDEVWDRAFEV
QTELIDRGSWRAVSMADLLIAATAERHGAIVLHYDGDYDMIRAVTAQPTQWVVPAGTAD

Nucleotide

• Length: 420 bp

>T105191 NZ_CP029190:c4622975-4622556 [Streptomyces venezuelae]
ATGAACACACAGCGCTACCTCATCGACAAGTCCGCCCTCGCCCGATATCCGCGCCCCGCAGTACGCGCCGTCCTCGACCC
CCTGCACCGAGCAGGGCTGCTCGCCGTCTGCGGGGCGGTCGAACTGGAAATCCTCTACAGCGCCCGGTCCAAGGCGGATG
CAGAACGGATCCGTGACGAGATGCGCGGATTCGAGTGGCTGCCCACACCGGATGAAGTCTGGGACCGGGCGTTCGAGGTT
CAGACCGAGCTCATCGACCGCGGCAGCTGGCGGGCGGTCTCCATGGCAGATCTCCTGATCGCGGCGACGGCCGAGCGCCA
CGGCGCCATCGTGCTGCACTACGACGGGGACTACGACATGATCCGCGCCGTTACGGCCCAGCCGACTCAGTGGGTGGTCC
CGGCCGGCACCGCCGACTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7598.58 Da
• Isoelectric Point: 4.3308

>AT105191 WP_150209451.1 NZ_CP029190:c4623187-4622972 [Streptomyces venezuelae]
MSRTVIDIDDKMLAEAAEIFGTTTKVATVNAALQDAVNRRKRESFLGWLAEGGLPDLTGPVKPEDEAQPAA

Nucleotide

• Length: 216 bp

>AT105191 NZ_CP029190:c4623187-4622972 [Streptomyces venezuelae]
ATGAGCCGCACCGTCATAGACATCGATGACAAGATGCTCGCCGAGGCAGCCGAGATCTTCGGCACGACCACCAAAGTCGC
CACGGTCAACGCCGCCCTCCAGGACGCGGTCAACCGCCGCAAGCGCGAATCGTTCCTCGGCTGGCTCGCAGAAGGCGGAT
TGCCGGACCTCACCGGACCGGTCAAGCCCGAAGACGAGGCCCAGCCGGCTGCATGA