Detailed information of TA system TA105175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 8899..9491
• Replicon: plasmid pMCR-H9
• Accession: NZ_CP029184
• Organism: Escherichia coli strain H9Ecoli

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1I9ZJC5
• Locus tag: DEF50_RS26145
• Protein ID: WP_000513532.1
• Coordinates: 8899..9147 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1I9ZJ49
• Locus tag: DEF50_RS26150
• Protein ID: WP_000681609.1
• Coordinates: 9144..9491 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DEF50_RS26100	3911..4576	-	666	WP_000532149.1	P-loop NTPase	-
DEF50_RS26110	5165..5494	+	330	WP_001542022.1	hypothetical protein	-
DEF50_RS26115	5895..6410	+	516	WP_001025393.1	J domain-containing protein	-
DEF50_RS26120	6514..7056	+	543	WP_001083904.1	hypothetical protein	-
DEF50_RS26125	7053..7307	+	255	WP_001230709.1	hypothetical protein	-
DEF50_RS26130	7304..7546	+	243	WP_000151456.1	hypothetical protein	-
DEF50_RS26140	8117..8410	+	294	WP_000673428.1	hypothetical protein	-
DEF50_RS26145	8899..9147	+	249	WP_000513532.1	type II toxin-antitoxin system HicA family toxin	Toxin
DEF50_RS26150	9144..9491	+	348	WP_000681609.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
DEF50_RS26155	9579..9761	-	183	WP_001428470.1	hypothetical protein	-
DEF50_RS26160	10263..10562	+	300	WP_034169410.1	type II toxin-antitoxin system HicB family antitoxin	-
DEF50_RS26165	10822..11148	-	327	WP_000206697.1	hypothetical protein	-
DEF50_RS26170	11150..11590	-	441	WP_023223352.1	hypothetical protein	-
DEF50_RS26175	11610..12317	-	708	WP_000108794.1	hypothetical protein	-
DEF50_RS26180	12319..13071	-	753	WP_000797966.1	conjugal transfer protein TraL	-
DEF50_RS26185	13089..13490	-	402	WP_021575837.1	hypothetical protein	-
DEF50_RS26190	13858..14199	+	342	WP_000730722.1	plasmid mobilization relaxosome protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..60942	60942

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9378.03 Da
• Isoelectric Point: 10.4800

>T105175 WP_000513532.1 NZ_CP029184:8899-9147 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Nucleotide

• Length: 249 bp

>T105175 NZ_CP029184:8899-9147 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13206.97 Da
• Isoelectric Point: 4.8313

>AT105175 WP_000681609.1 NZ_CP029184:9144-9491 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Nucleotide

• Length: 348 bp

>AT105175 NZ_CP029184:9144-9491 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGAAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A1I9ZJC5

Antitoxin

• AlphaFold DB: A0A1I9ZJ49