Detailed information of TA system TA105174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 7053..7546
• Replicon: plasmid pMCR-H9
• Accession: NZ_CP029184
• Organism: Escherichia coli strain H9Ecoli

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9Y9W8
• Locus tag: DEF50_RS26125
• Protein ID: WP_001230709.1
• Coordinates: 7053..7307 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: U9Y4R8
• Locus tag: DEF50_RS26130
• Protein ID: WP_000151456.1
• Coordinates: 7304..7546 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DEF50_RS26085	2628..2789	+	162	WP_001366886.1	type I toxin-antitoxin system Hok family toxin	-
DEF50_RS26090	2939..3535	+	597	WP_000125165.1	hypothetical protein	-
DEF50_RS26870	3553..3837	-	285	WP_001162371.1	hypothetical protein	-
DEF50_RS26100	3911..4576	-	666	WP_000532149.1	P-loop NTPase	-
DEF50_RS26110	5165..5494	+	330	WP_001542022.1	hypothetical protein	-
DEF50_RS26115	5895..6410	+	516	WP_001025393.1	J domain-containing protein	-
DEF50_RS26120	6514..7056	+	543	WP_001083904.1	hypothetical protein	-
DEF50_RS26125	7053..7307	+	255	WP_001230709.1	hypothetical protein	Toxin
DEF50_RS26130	7304..7546	+	243	WP_000151456.1	hypothetical protein	Antitoxin
DEF50_RS26140	8117..8410	+	294	WP_000673428.1	hypothetical protein	-
DEF50_RS26145	8899..9147	+	249	WP_000513532.1	type II toxin-antitoxin system HicA family toxin	-
DEF50_RS26150	9144..9491	+	348	WP_000681609.1	type II toxin-antitoxin system HicB family antitoxin	-
DEF50_RS26155	9579..9761	-	183	WP_001428470.1	hypothetical protein	-
DEF50_RS26160	10263..10562	+	300	WP_034169410.1	type II toxin-antitoxin system HicB family antitoxin	-
DEF50_RS26165	10822..11148	-	327	WP_000206697.1	hypothetical protein	-
DEF50_RS26170	11150..11590	-	441	WP_023223352.1	hypothetical protein	-
DEF50_RS26175	11610..12317	-	708	WP_000108794.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..60942	60942

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9942.47 Da
• Isoelectric Point: 10.1206

>T105174 WP_001230709.1 NZ_CP029184:7053-7307 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Nucleotide

• Length: 255 bp

>T105174 NZ_CP029184:7053-7307 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9136.45 Da
• Isoelectric Point: 7.2775

>AT105174 WP_000151456.1 NZ_CP029184:7304-7546 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Nucleotide

• Length: 243 bp

>AT105174 NZ_CP029184:7304-7546 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Structures

Toxin

• AlphaFold DB: A0A3G9H8F0

Antitoxin

• AlphaFold DB: A0A3G9GNF5