Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 874..1396 Replicon plasmid p3-H9
Accession NZ_CP029182
Organism Escherichia coli strain H9Ecoli

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag DEF50_RS25845 Protein ID WP_000220561.1
Coordinates 874..1155 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag DEF50_RS25850 Protein ID WP_000121743.1
Coordinates 1145..1396 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DEF50_RS26695 543..635 + 93 Protein_1 IS6 family transposase -
DEF50_RS25845 874..1155 - 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DEF50_RS25850 1145..1396 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
DEF50_RS25860 2631..3470 + 840 WP_024129959.1 replication initiation protein -
DEF50_RS25865 3491..4333 + 843 WP_000435064.1 replication initiation protein -
DEF50_RS25870 4337..4540 + 204 Protein_6 hypothetical protein -
DEF50_RS25875 4595..5299 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
DEF50_RS25880 5363..5599 + 237 Protein_8 DNA distortion polypeptide 3 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(3)-IId / mph(A) / aph(3')-IIa - 1..31250 31250
- inside IScluster/Tn - - 111..5299 5188


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T105173 WP_000220561.1 NZ_CP029182:c1155-874 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T105173 NZ_CP029182:c1155-874 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT105173 WP_000121743.1 NZ_CP029182:c1396-1145 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT105173 NZ_CP029182:c1396-1145 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References