Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 105073..105674 Replicon plasmid p1-H9
Accession NZ_CP029181
Organism Escherichia coli strain H9Ecoli

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag DEF50_RS25620 Protein ID WP_001216034.1
Coordinates 105073..105453 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A826P616
Locus tag DEF50_RS25625 Protein ID WP_059039668.1
Coordinates 105453..105674 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DEF50_RS25590 101447..102626 - 1180 Protein_105 terminase -
DEF50_RS25595 102713..103165 - 453 WP_001312282.1 late promoter-activating protein (Gp10) -
DEF50_RS25600 103230..103927 + 698 Protein_107 IS1 family transposase -
DEF50_RS25610 103944..104861 - 918 Protein_108 DUF968 domain-containing protein -
DEF50_RS25615 104889..105068 - 180 WP_038997082.1 hypothetical protein -
DEF50_RS25620 105073..105453 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
DEF50_RS25625 105453..105674 - 222 WP_059039668.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
DEF50_RS25630 105857..107413 + 1557 WP_038997084.1 type I restriction-modification system subunit M -
DEF50_RS25635 107410..108636 + 1227 Protein_113 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid oqxA / oqxB / qacE / ant(3'')-Ia / dfrA1 / tet(A) - 1..145524 145524
- flank IS/Tn - - 103424..103927 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T105172 WP_001216034.1 NZ_CP029181:c105453-105073 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T105172 NZ_CP029181:c105453-105073 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGCATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8147.13 Da        Isoelectric Point: 4.7708

>AT105172 WP_059039668.1 NZ_CP029181:c105674-105453 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEIEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT105172 NZ_CP029181:c105674-105453 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGATTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A826P616

References