Detailed information of TA system TA105171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 47603..48138
• Replicon: plasmid p1-H9
• Accession: NZ_CP029181
• Organism: Escherichia coli strain H9Ecoli

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: DEF50_RS25260
• Protein ID: WP_032206513.1
• Coordinates: 47603..47890 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: T1PXV2
• Locus tag: DEF50_RS25265
• Protein ID: WP_001139206.1
• Coordinates: 47887..48138 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DEF50_RS25245	43228..46743	+	3516	WP_089571546.1	autotransporter outer membrane beta-barrel domain-containing protein	-
DEF50_RS25250	46918..47123	-	206	Protein_44	hypothetical protein	-
DEF50_RS25255	47113..47313	+	201	WP_000896262.1	DUF839 domain-containing protein	-
DEF50_RS25260	47603..47890	-	288	WP_032206513.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DEF50_RS25265	47887..48138	-	252	WP_001139206.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DEF50_RS25275	48721..49884	+	1164	Protein_48	helicase	-
DEF50_RS25280	50074..51090	-	1017	Protein_49	methyl-accepting chemotaxis protein	-
DEF50_RS25290	51134..51838	-	705	WP_032236663.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	oqxA / oqxB / qacE / ant(3'')-Ia / dfrA1 / tet(A)	-	1..145524	145524

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11130.20 Da
• Isoelectric Point: 10.5833

>T105171 WP_032206513.1 NZ_CP029181:c47890-47603 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKTIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQIIDDMLIIAVVAVGK
RERSNVYHLASERMK

Nucleotide

• Length: 288 bp

>T105171 NZ_CP029181:c47890-47603 [Escherichia coli]
ATGACTTATACAGTAAAATTCCGGGATGATGCACTGAAAGAATGGCTCAGACTGGACAAGACCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCTCATATAGCTTCTGCAAAACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTTTATCAGATTATTGATGATATGTTAATTATTGCGGTCGTCGCAGTAGGTAAA
CGTGAACGCAGCAACGTTTATCATCTCGCCAGTGAACGGATGAAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9171.39 Da
• Isoelectric Point: 4.0927

>AT105171 WP_001139206.1 NZ_CP029181:c48138-47887 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT105171 NZ_CP029181:c48138-47887 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAGCTGAAAAAAAATCCGATGGCAACTGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCAGCATTCTACTGTGTCCCTGCTGAGCTCTATGAAAGAATGC
TTGATGCCTTGGATGACCAGGAGCTGGTAAAACTGGTTACTGAACGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Antitoxin

• AlphaFold DB: T1PXV2