Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 3601522..3602117 Replicon chromosome
Accession NZ_CP029180
Organism Escherichia coli strain H9Ecoli

Toxin (Protein)


Gene name chpB Uniprot ID A0A827ISR3
Locus tag DEF50_RS19090 Protein ID WP_061359519.1
Coordinates 3601767..3602117 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID A0A240D6C8
Locus tag DEF50_RS19085 Protein ID WP_001223212.1
Coordinates 3601522..3601773 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DEF50_RS19075 3597187..3600966 + 3780 WP_061359518.1 autotransporter assembly complex protein TamB -
DEF50_RS19080 3600969..3601310 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
DEF50_RS19085 3601522..3601773 + 252 WP_001223212.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
DEF50_RS19090 3601767..3602117 + 351 WP_061359519.1 endoribonuclease toxin ChpB Toxin
DEF50_RS19095 3602194..3602724 - 531 WP_000055070.1 inorganic diphosphatase -
DEF50_RS19100 3603034..3603990 + 957 WP_000265942.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
DEF50_RS19105 3604130..3605632 + 1503 WP_072645310.1 sugar ABC transporter ATP-binding protein -
DEF50_RS19110 3605646..3606668 + 1023 WP_001301928.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-114)

Antitoxin

Antitoxin PemI-like

(1-83)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12506.43 Da        Isoelectric Point: 6.4786

>T105167 WP_061359519.1 NZ_CP029180:3601767-3602117 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLHARQAKRIGLAADEVVEEALLRLQAVVK

Download         Length: 351 bp

>T105167 NZ_CP029180:3601767-3602117 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCAGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGAAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9301.61 Da        Isoelectric Point: 4.6509

>AT105167 WP_001223212.1 NZ_CP029180:3601522-3601773 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT105167 NZ_CP029180:3601522-3601773 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCTACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A827ISR3

Antitoxin

Source ID Structure
AlphaFold DB A0A240D6C8

References