Detailed information of TA system TA105150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 22750..23330
• Replicon: plasmid unnamed2
• Accession: NZ_CP029178
• Organism: Acidibrevibacterium fodinaquatile strain G45-3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DEF76_RS18930
• Protein ID: WP_114914072.1
• Coordinates: 22965..23330 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DEF76_RS18925
• Protein ID: WP_162800782.1
• Coordinates: 22750..22968 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DEF76_RS18900	18399..18731	+	333	WP_114914068.1	hypothetical protein	-
DEF76_RS18905	19254..19838	-	585	WP_114911760.1	ISAs1 family transposase	-
DEF76_RS18910	19790..20380	-	591	WP_162800530.1	ISAs1 family transposase	-
DEF76_RS19590	20599..20838	+	240	WP_162800776.1	hypothetical protein	-
DEF76_RS18915	21493..21891	-	399	WP_114914069.1	type II toxin-antitoxin system VapC family toxin	-
DEF76_RS18920	21888..22154	-	267	WP_114914070.1	transcriptional regulator	-
DEF76_RS18925	22750..22968	+	219	WP_162800782.1	antitoxin MazE family protein	Antitoxin
DEF76_RS18930	22965..23330	+	366	WP_114914072.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DEF76_RS18935	24046..25170	-	1125	WP_114914073.1	replication initiator protein A	-
DEF76_RS18945	25539..26174	-	636	WP_114914075.1	ParA family protein	-
DEF76_RS18950	26510..26884	+	375	WP_114914076.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DEF76_RS18955	26877..27194	+	318	WP_114914077.1	DNA-binding transcriptional regulator	-
DEF76_RS18960	27311..27598	-	288	WP_114914078.1	type II toxin-antitoxin system ParD family antitoxin	-
DEF76_RS18965	27696..28274	-	579	WP_114914079.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..45771	45771

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-107)

Antitoxin

• Antitoxin MazE-like (8-70)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13513.55 Da
• Isoelectric Point: 10.0962

>T105150 WP_114914072.1 NZ_CP029178:22965-23330 [Acidibrevibacterium fodinaquatile]
MRRGEMWTVAGGKDYAGKPRPVVIVQDDAFDATDSITICAFTTDPTEAPLFRLSVESNERNGLRSSSRLMVDKITTVPKS
KVGKRVGRLDDEDIIRLNQAMMVFLGLTVSPRIGKKWNNEK

Nucleotide

• Length: 366 bp

>T105150 NZ_CP029178:22965-23330 [Acidibrevibacterium fodinaquatile]
ATGAGACGGGGCGAGATGTGGACCGTCGCCGGCGGCAAGGACTACGCGGGTAAGCCGCGGCCGGTCGTTATCGTGCAAGA
CGATGCCTTCGACGCGACAGATTCCATCACCATCTGCGCCTTCACAACGGACCCGACCGAGGCTCCTTTGTTCCGGCTAT
CGGTGGAATCGAACGAACGCAACGGGCTGCGTTCCTCCTCCCGCCTAATGGTGGACAAAATTACCACCGTCCCGAAGTCC
AAGGTTGGTAAGCGGGTCGGACGGTTGGACGATGAAGACATAATTCGGCTTAACCAGGCCATGATGGTGTTCCTGGGCCT
CACGGTATCGCCAAGAATAGGTAAAAAATGGAATAATGAAAAATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8094.09 Da
• Isoelectric Point: 10.6827

>AT105150 WP_162800782.1 NZ_CP029178:22750-22968 [Acidibrevibacterium fodinaquatile]
MRGAKPTRVKVQEHRNRLRAQGLRPIQIWVPDVRASFFRAEAHRQSLAVAASAHAAEDQAFIDAVSSDWGEE

Nucleotide

• Length: 219 bp

>AT105150 NZ_CP029178:22750-22968 [Acidibrevibacterium fodinaquatile]
ATGAGAGGCGCGAAGCCCACCAGGGTAAAGGTGCAGGAGCATCGCAACCGGCTGCGTGCCCAGGGCCTGCGGCCAATTCA
GATTTGGGTGCCGGATGTCCGGGCGTCGTTTTTTCGTGCGGAAGCACATCGACAATCCCTGGCCGTGGCAGCGAGTGCCC
ATGCGGCCGAGGATCAAGCCTTCATCGATGCGGTCTCCTCCGATTGGGGCGAGGAATGA