Detailed information of TA system TA105130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 78722..79830
• Replicon: chromosome
• Accession: NZ_CP029172
• Organism: Staphylococcus aureus strain PTDrAP2

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: -
• Locus tag: DD562_RS00475
• Protein ID: WP_109028163.1
• Coordinates: 78961..79830 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: DD562_RS00470
• Protein ID: WP_000205227.1
• Coordinates: 78722..78946 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DD562_RS00450	74217..75581	+	1365	WP_037537719.1	YqeG family HAD IIIA-type phosphatase	-
DD562_RS00455	76302..76598	+	297	WP_002360685.1	hypothetical protein	-
DD562_RS00460	76599..77015	+	417	WP_000323438.1	recombinase	-
DD562_RS00465	77035..78579	+	1545	WP_002390960.1	recombinase family protein	-
DD562_RS00470	78722..78946	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
DD562_RS00475	78961..79830	+	870	WP_109028163.1	nucleotidyltransferase domain-containing protein	Toxin
DD562_RS00480	79811..80545	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
DD562_RS00485	80578..81441	+	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
DD562_RS00490	81485..82012	+	528	WP_002294507.1	adenine phosphoribosyltransferase	-
DD562_RS00495	82144..82953	+	810	WP_002294509.1	ANT(9) family aminoglycoside nucleotidyltransferase Spw	-
DD562_RS00500	83171..83422	+	252	WP_002294510.1	hypothetical protein	-
DD562_RS00505	83473..83931	-	459	WP_002325145.1	hypothetical protein	-
DD562_RS00510	84017..84577	+	561	Protein_77	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	mecA / tet(K) / ant(6)-Ia / lsa(E) / lnu(B) / vga(A)V	adsA	27135..144348	117213
	-	inside	SCCmec	mecA / tet(K) / ant(6)-Ia / lsa(E) / lnu(B)	-	40521..106130	65609
	-	flank	IS/Tn	ant(6)-Ia / lsa(E) / lnu(B)	-	80578..90860	10282

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32925.63 Da
• Isoelectric Point: 5.0903

>T105130 WP_109028163.1 NZ_CP029172:78961-79830 [Staphylococcus aureus]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINKKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T105130 NZ_CP029172:78961-79830 [Staphylococcus aureus]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATCGTATTAGGGGGCTC
ACGTGCAAGAGGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAATTGGATGATAAAAATAGAAACAACCTTGTTGTGCCTCCCGGAGCGTGGGGTGATTGG
ATTAATGGCGGCGGATGGTTATTTATAAATGGGTATCATGTTGACTTGATTTTACGTGATATAAAACGGGTAGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCAATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGTAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACAAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGTTACGATATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT105130 WP_000205227.1 NZ_CP029172:78722-78946 [Staphylococcus aureus]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT105130 NZ_CP029172:78722-78946 [Staphylococcus aureus]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTCAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGAAGAAATAAAAATATCTAACGAATAA