Detailed information of TA system TA105120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2227831..2228360
• Replicon: chromosome
• Accession: NZ_CP029166
• Organism: Staphylococcus aureus strain SVH7513

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DD555_RS11780
• Protein ID: WP_000621175.1
• Coordinates: 2227831..2228193 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: DD555_RS11785
• Protein ID: WP_000948331.1
• Coordinates: 2228190..2228360 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DD555_RS11755	2224809..2225579	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
DD555_RS11760	2225554..2226033	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
DD555_RS11765	2226035..2226361	-	327	WP_001052491.1	anti-sigma factor antagonist	-
DD555_RS11770	2226480..2227481	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
DD555_RS11780	2227831..2228193	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DD555_RS11785	2228190..2228360	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
DD555_RS11790	2228445..2229593	-	1149	WP_001281145.1	alanine racemase	-
DD555_RS11795	2229659..2230018	-	360	WP_000581200.1	holo-ACP synthase	-
DD555_RS11800	2230022..2230513	-	492	WP_001205910.1	PH domain-containing protein	-
DD555_RS11805	2230500..2232083	-	1584	WP_001294626.1	PH domain-containing protein	-
DD555_RS11810	2232076..2232555	-	480	WP_001287088.1	hypothetical protein	-
DD555_RS11815	2232763..2233323	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T105120 WP_000621175.1 NZ_CP029166:c2228193-2227831 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T105120 NZ_CP029166:c2228193-2227831 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT105120 WP_000948331.1 NZ_CP029166:c2228360-2228190 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT105120 NZ_CP029166:c2228360-2228190 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1