Detailed information of TA system TA105105

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4692802..4693600
• Replicon: chromosome
• Accession: NZ_CP029164
• Organism: Escherichia coli strain 104

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q8VRA6
• Locus tag: DDV95_RS24255
• Protein ID: WP_000854730.1
• Coordinates: 4693223..4693600 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0P0SQV8
• Locus tag: DDV95_RS24250
• Protein ID: WP_001285481.1
• Coordinates: 4692802..4693176 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDV95_RS24210	4688777..4689229	+	453	WP_000682723.1	hypothetical protein	-
DDV95_RS24215	4689347..4689580	+	234	WP_001213776.1	DUF905 family protein	-
DDV95_RS24220	4689680..4690501	+	822	WP_001234359.1	DUF945 domain-containing protein	-
DDV95_RS24225	4690501..4690746	+	246	WP_001164966.1	hypothetical protein	-
DDV95_RS24230	4690840..4691313	+	474	WP_001313575.1	antirestriction protein	-
DDV95_RS24235	4691329..4691805	+	477	WP_001313574.1	RadC family protein	-
DDV95_RS24240	4691868..4692089	+	222	WP_000692315.1	DUF987 domain-containing protein	-
DDV95_RS24245	4692108..4692752	+	645	WP_000086759.1	hypothetical protein	-
DDV95_RS24250	4692802..4693176	+	375	WP_001285481.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DDV95_RS24255	4693223..4693600	+	378	WP_000854730.1	TA system toxin CbtA family protein	Toxin
DDV95_RS24260	4693597..4694089	+	493	Protein_4417	hypothetical protein	-
DDV95_RS24265	4694168..4695156	-	989	Protein_4418	IS630 family transposase	-
DDV95_RS24270	4695304..4696897	-	1594	Protein_4419	IS66 family transposase	-
DDV95_RS24275	4696928..4697278	-	351	WP_000624707.1	IS66 family insertion sequence element accessory protein TnpB	-
DDV95_RS24280	4697275..4697566	-	292	Protein_4421	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14212.21 Da
• Isoelectric Point: 7.2923

>T105105 WP_000854730.1 NZ_CP029164:4693223-4693600 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRMVNDIIRGEHSEAKR

Nucleotide

• Length: 378 bp

>T105105 NZ_CP029164:4693223-4693600 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCTTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13817.53 Da
• Isoelectric Point: 4.7511

>AT105105 WP_001285481.1 NZ_CP029164:4692802-4693176 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTIG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT105105 NZ_CP029164:4692802-4693176 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCATCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A0H2V671

Antitoxin

• AlphaFold DB: A0A0P0SQV8