Detailed information of TA system TA105100

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3394191..3394827
• Replicon: chromosome
• Accession: NZ_CP029164
• Organism: Escherichia coli strain 104

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A5F1EUB3
• Locus tag: DDV95_RS17395
• Protein ID: WP_000813796.1
• Coordinates: 3394651..3394827 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: DDV95_RS17390
• Protein ID: WP_076838470.1
• Coordinates: 3394191..3394607 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DDV95_RS17370	3389343..3390284	-	942	WP_086525100.1	ABC transporter permease	-
DDV95_RS17375	3390285..3391298	-	1014	WP_000220433.1	ABC transporter ATP-binding protein	-
DDV95_RS17380	3391316..3392461	-	1146	WP_000047448.1	ABC transporter substrate-binding protein	-
DDV95_RS17385	3392706..3394112	-	1407	WP_000760605.1	PLP-dependent aminotransferase family protein	-
DDV95_RS17390	3394191..3394607	-	417	WP_076838470.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
DDV95_RS17395	3394651..3394827	-	177	WP_000813796.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
DDV95_RS17400	3395049..3395279	+	231	WP_000494243.1	YncJ family protein	-
DDV95_RS17405	3395371..3397332	-	1962	WP_001317809.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
DDV95_RS17410	3397405..3397941	-	537	WP_000429147.1	DNA-binding transcriptional regulator SutR	-
DDV95_RS17415	3397994..3399205	+	1212	WP_071593090.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6751.78 Da
• Isoelectric Point: 11.5666

>T105100 WP_000813796.1 NZ_CP029164:c3394827-3394651 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLN

Nucleotide

• Length: 177 bp

>T105100 NZ_CP029164:c3394827-3394651 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTAAATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15219.59 Da
• Isoelectric Point: 4.5908

>AT105100 WP_076838470.1 NZ_CP029164:c3394607-3394191 [Escherichia coli]
MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT105100 NZ_CP029164:c3394607-3394191 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACTCCTGCGCCGGAAGGCGGTTATGTGGTTTCTTTTGTAGATATCCCTGAAGCGTTGAC
GCAGGGAGAAACTGTTGCTGAAGCGATGGAAGCAGCAAAAGATGCCTTACTCACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCTTATTTAACTTGCACCATGCTACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Structures

Toxin

• AlphaFold DB: A0A5F1EUB3