Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 4385289..4385923 Replicon chromosome
Accession NZ_CP029159
Organism Streptomyces tsukubensis NRRL18488

Toxin (Protein)


Gene name vapC Uniprot ID I2N1L9
Locus tag STSU_RS18310 Protein ID WP_006348208.1
Coordinates 4385289..4385711 (-) Length 141 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A7G3UFR9
Locus tag STSU_RS18315 Protein ID WP_040915655.1
Coordinates 4385708..4385923 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
STSU_RS18280 4380730..4381548 + 819 WP_006348202.1 DUF817 domain-containing protein -
STSU_RS18285 4381638..4382111 + 474 WP_006348203.1 carboxymuconolactone decarboxylase family protein -
STSU_RS18290 4382253..4382585 - 333 WP_198965075.1 cupin domain-containing protein -
STSU_RS18295 4382746..4383078 - 333 WP_006348205.1 helix-turn-helix transcriptional regulator -
STSU_RS18300 4383078..4383374 - 297 WP_078902262.1 type II toxin-antitoxin system RelE/ParE family toxin -
STSU_RS18305 4383528..4384832 - 1305 WP_006348207.1 cation:proton antiporter -
STSU_RS18310 4385289..4385711 - 423 WP_006348208.1 PIN domain nuclease Toxin
STSU_RS18315 4385708..4385923 - 216 WP_040915655.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
STSU_RS18320 4386367..4387026 + 660 WP_040915657.1 HAD family hydrolase -
STSU_RS18325 4387007..4388281 - 1275 WP_040915674.1 hypothetical protein -
STSU_RS18330 4388619..4388978 + 360 WP_006348212.1 hypothetical protein -
STSU_RS18335 4389074..4390285 + 1212 WP_040915676.1 radical SAM protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-124)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(3-46)


Sequences

Toxin        


Download         Length: 141 a.a.        Molecular weight: 15468.37 Da        Isoelectric Point: 4.5876

>T105076 WP_006348208.1 NZ_CP029159:c4385711-4385289 [Streptomyces tsukubensis NRRL18488]
VSAAQFLIDTSALARFLRGDAEQYGWDQAAAAGLIATCPITELEFFYSARSAEDRARGVEDLRMIFGWVPVDDRAYDRAW
QVQELLTRRGQHRSAGPVDLVVAATAELQGLTLLHRDRDFDCIAAVTGQALQWFGPDPGK

Download         Length: 423 bp

>T105076 NZ_CP029159:c4385711-4385289 [Streptomyces tsukubensis NRRL18488]
GTGAGCGCGGCGCAGTTTCTGATCGACACCAGCGCGCTCGCCCGATTCCTCCGCGGGGACGCCGAGCAGTACGGCTGGGA
CCAGGCAGCAGCAGCCGGGCTCATCGCGACCTGCCCCATCACCGAGCTGGAGTTCTTCTACAGCGCGCGATCAGCCGAGG
ATCGCGCCCGGGGCGTCGAGGATCTGCGGATGATCTTCGGCTGGGTCCCCGTCGACGACCGCGCCTACGATCGCGCCTGG
CAGGTCCAGGAACTGCTGACTCGGCGCGGACAGCACCGCAGCGCCGGCCCGGTTGACCTCGTCGTCGCCGCCACAGCGGA
GTTGCAGGGCCTCACCCTCCTGCACCGCGACCGCGATTTCGACTGCATCGCCGCCGTCACCGGTCAGGCCCTGCAGTGGT
TCGGCCCCGATCCGGGCAAATGA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7893.87 Da        Isoelectric Point: 5.0634

>AT105076 WP_040915655.1 NZ_CP029159:c4385923-4385708 [Streptomyces tsukubensis NRRL18488]
MSRTVIDLDDELLADVAQALGTSTKKETVNTALREVLENRRRALALARLRAAAADGGFDLDVFDDKQSYRR

Download         Length: 216 bp

>AT105076 NZ_CP029159:c4385923-4385708 [Streptomyces tsukubensis NRRL18488]
ATGAGTCGCACAGTGATCGACCTGGACGACGAGCTGCTTGCCGACGTGGCGCAGGCGCTGGGAACCAGCACCAAGAAGGA
GACGGTCAATACCGCCCTGCGGGAAGTACTGGAGAACCGGCGCCGGGCTCTCGCTCTGGCCCGGCTGCGCGCGGCGGCGG
CCGACGGGGGCTTCGACCTGGACGTCTTCGACGACAAGCAGAGCTACCGGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I2N1L9

Antitoxin

Source ID Structure
AlphaFold DB A0A7G3UFR9

References