Detailed information of TA system TA105054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1997708..1999257
• Replicon: chromosome
• Accession: NZ_CP029150
• Organism: Glaesserella parasuis strain GZ20170512

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: C3363_RS09935
• Protein ID: WP_159179995.1
• Coordinates: 1997708..1999024 (-)
• Length: 439 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: U4SAZ6
• Locus tag: C3363_RS09940
• Protein ID: WP_010786623.1
• Coordinates: 1999018..1999257 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3363_RS09895	1992808..1993395	+	588	WP_010787333.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
C3363_RS09900	1993651..1993830	-	180	Protein_1906	disulfide bond formation protein DsbA	-
C3363_RS09905	1993830..1994273	-	444	WP_015939865.1	nuclear transport factor 2 family protein	-
C3363_RS09910	1994408..1994524	+	117	Protein_1908	LysR family transcriptional regulator	-
C3363_RS09915	1994605..1995178	-	574	Protein_1909	virulence RhuM family protein	-
C3363_RS09920	1995365..1995724	-	360	WP_010787368.1	hypothetical protein	-
C3363_RS09925	1995624..1996139	+	516	WP_080513259.1	hypothetical protein	-
C3363_RS09930	1996163..1997704	+	1542	WP_201288378.1	PLP-dependent aminotransferase family protein	-
C3363_RS09935	1997708..1999024	-	1317	WP_159179995.1	type II toxin-antitoxin system HipA family toxin	Toxin
C3363_RS09940	1999018..1999257	-	240	WP_010786623.1	helix-turn-helix domain-containing protein	Antitoxin
C3363_RS09945	1999404..2000231	-	828	WP_010786622.1	alpha/beta hydrolase	-
C3363_RS09950	2000362..2001808	+	1447	Protein_1916	metalloprotease TldD	-
C3363_RS09955	2001995..2003092	-	1098	WP_020997155.1	ISAs1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (151-404)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-66)

Sequences

Toxin

Protein

• Length: 439 a.a.
• Molecular weight: 49971.25 Da
• Isoelectric Point: 7.6047

>T105054 WP_159179995.1 NZ_CP029150:c1999024-1997708 [Glaesserella parasuis]
MVMRLRVLMNGEMVGYFDRTLSGSHHFTYAESWLNSPLARPISLSMPLQRATYQGKEVINFFDNLLPDNPQIRARIATRF
NAETQQPFDLLAKIGRDAAGAITLLGEDEQIPNHRIIQATELNESEMAKILQSYQYNAPLGMIDNNQDFRISIAGAQEKT
AMLWHNGKWKLPHGLTPTTHIFKLPMGEIRTPKHLLDLSKSVDNELVCLRLFSAFNIPSANVELLEMNDIRALAVERFDR
RLSQDGSYFLRLPYEDFCQAFGLSPTQKYESDGGVGIGDMMNKLLYSSNPNDRRQFMQTQFLFWLLAAIDGHAKNFSLAI
EKQGRYHLSPVYDVLSAYPMLGGKGWHHSELQMAMSLRSTQKGKKWHWSKIYAEHFYATAKFVGFSSSDMKQVIEQICDT
VQETIDSVATTLPKQIDENVRDQIFTGIMKQVQKVSMK

Nucleotide

• Length: 1317 bp

>T105054 NZ_CP029150:c1999024-1997708 [Glaesserella parasuis]
ATGGTAATGCGATTACGAGTATTAATGAATGGCGAAATGGTTGGGTATTTTGACCGCACTTTATCGGGTTCCCACCATTT
TACCTATGCTGAAAGTTGGCTAAATTCCCCATTAGCTCGCCCGATTTCCCTTTCTATGCCCCTGCAAAGAGCAACATATC
AAGGCAAAGAAGTGATTAATTTCTTTGATAATTTACTGCCCGATAATCCTCAAATTCGAGCAAGAATTGCGACAAGATTT
AATGCTGAAACACAACAACCTTTTGACTTACTTGCTAAAATTGGTCGCGATGCTGCGGGTGCTATTACTCTTTTAGGGGA
AGATGAACAAATTCCTAATCATCGAATAATTCAAGCCACAGAATTAAACGAGAGTGAAATGGCGAAAATTCTACAAAGTT
ATCAATACAATGCCCCGCTTGGTATGATTGATAATAATCAGGACTTCAGAATTTCTATTGCTGGTGCACAAGAAAAAACA
GCTATGCTTTGGCATAACGGCAAATGGAAATTACCACACGGATTGACCCCGACAACGCATATTTTCAAATTGCCTATGGG
AGAAATTCGTACCCCTAAACACCTGCTTGATCTGTCAAAAAGTGTAGATAACGAACTCGTCTGTTTGAGATTATTTTCTG
CTTTCAATATCCCTTCCGCAAACGTTGAATTATTGGAAATGAACGATATACGGGCATTAGCTGTTGAACGTTTTGATCGC
CGTCTTAGTCAAGATGGCAGTTATTTTTTACGTTTACCTTACGAAGATTTCTGTCAGGCATTCGGCTTATCGCCAACGCA
AAAATATGAAAGCGATGGTGGAGTTGGTATTGGGGATATGATGAATAAGTTGCTCTACTCCAGTAATCCCAACGATCGCC
GTCAATTTATGCAAACTCAATTTCTGTTTTGGCTACTGGCTGCCATTGATGGACATGCTAAAAACTTTTCTCTCGCCATT
GAAAAACAAGGGCGTTATCACTTATCCCCAGTGTATGACGTACTTTCTGCTTATCCAATGTTAGGCGGAAAAGGTTGGCA
TCACAGTGAATTACAAATGGCAATGAGCTTGCGTAGCACACAAAAAGGAAAAAAATGGCATTGGAGTAAAATTTATGCTG
AACATTTCTATGCCACAGCTAAGTTTGTCGGATTTAGCTCAAGTGATATGAAACAAGTGATAGAGCAAATTTGCGATACA
GTGCAAGAAACCATTGATAGCGTAGCAACAACACTACCCAAACAGATAGATGAAAATGTACGGGATCAAATATTCACGGG
CATAATGAAACAGGTGCAAAAAGTTTCTATGAAGTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9059.47 Da
• Isoelectric Point: 9.7684

>AT105054 WP_010786623.1 NZ_CP029150:c1999257-1999018 [Glaesserella parasuis]
MLITTPDMLGNSLRDIRKQKKLSQMQIANQVALRQATISTFEKKTDKATIETLFKLLSGLGYELHIQPRPEKTEGNDEW

Nucleotide

• Length: 240 bp

>AT105054 NZ_CP029150:c1999257-1999018 [Glaesserella parasuis]
ATGTTAATCACGACACCAGATATGCTTGGAAATAGCCTACGCGATATTCGTAAGCAAAAAAAGCTTTCTCAAATGCAAAT
AGCAAATCAAGTAGCTTTAAGACAAGCCACGATTTCCACCTTTGAGAAAAAAACCGATAAAGCCACTATTGAAACATTAT
TCAAGTTACTTTCAGGTTTAGGCTATGAACTACATATCCAACCTCGTCCTGAAAAAACAGAGGGGAACGATGAATGGTAA

Structures

Antitoxin

• AlphaFold DB: U4SAZ6