Detailed information of TA system TA105046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 4257373..4257938
• Replicon: chromosome
• Accession: NZ_CP029146
• Organism: Rhodococcus ruber strain SD3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A098BGQ9
• Locus tag: DCN13_RS19320
• Protein ID: WP_040270293.1
• Coordinates: 4257373..4257729 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A8D4K486
• Locus tag: DCN13_RS19325
• Protein ID: WP_040270291.1
• Coordinates: 4257726..4257938 (-)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DCN13_RS19305	4252635..4254563	-	1929	WP_006554649.1	tyrosine-type recombinase/integrase	-
DCN13_RS19310	4255160..4256347	-	1188	WP_061064897.1	tyrosine-type recombinase/integrase	-
DCN13_RS19315	4256471..4257298	+	828	WP_059174977.1	hypothetical protein	-
DCN13_RS19320	4257373..4257729	-	357	WP_040270293.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DCN13_RS19325	4257726..4257938	-	213	WP_040270291.1	ribbon-helix-helix protein, CopG family	Antitoxin
DCN13_RS19330	4258148..4258729	+	582	WP_040270289.1	GNAT family N-acetyltransferase	-
DCN13_RS19335	4258737..4259768	-	1032	WP_040270287.1	hypothetical protein	-
DCN13_RS19340	4259882..4260856	-	975	WP_040270285.1	helix-turn-helix domain-containing protein	-
DCN13_RS19345	4260993..4261631	+	639	WP_064065754.1	isochorismatase family protein	-
DCN13_RS19350	4261768..4262679	+	912	WP_017679961.1	SUMF1/EgtB/PvdO family nonheme iron enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-114)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-40)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12780.56 Da
• Isoelectric Point: 7.3225

>T105046 WP_040270293.1 NZ_CP029146:c4257729-4257373 [Rhodococcus ruber]
MIRRAQIHWVDLDPAQGGEAAERRPAVVVSNDRANATAENLGRGVVTVVPLIASTSHVFPFQVLVEPDESGLTRASKAQA
EQVRSVSVRRLDAEPVGKLGVRTLAALDEALRLHLDLR

Nucleotide

• Length: 357 bp

>T105046 NZ_CP029146:c4257729-4257373 [Rhodococcus ruber]
ATGATCCGCCGAGCGCAGATCCACTGGGTCGATCTGGACCCGGCGCAGGGTGGCGAAGCGGCCGAGCGTCGGCCGGCAGT
GGTGGTAAGCAACGACCGCGCCAACGCGACCGCCGAGAACCTGGGCAGAGGAGTGGTCACCGTCGTTCCTCTGATCGCCA
GCACCTCACACGTCTTTCCGTTCCAGGTACTCGTGGAACCCGACGAGTCGGGTCTGACCAGAGCGTCGAAAGCTCAGGCA
GAACAGGTACGTTCGGTGTCCGTGCGCCGCCTGGACGCCGAACCTGTCGGAAAACTCGGGGTGAGAACACTCGCAGCGCT
CGACGAAGCTCTACGGCTGCATCTCGATCTGCGCTGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7680.46 Da
• Isoelectric Point: 4.1694

>AT105046 WP_040270291.1 NZ_CP029146:c4257938-4257726 [Rhodococcus ruber]
MTVKFTISLPDEDVHVLDEYIRDHGLRGRSAGVRAAVRLLAAAGLERDYATAFAEVDTDDAWDVTTADTE

Nucleotide

• Length: 213 bp

>AT105046 NZ_CP029146:c4257938-4257726 [Rhodococcus ruber]
ATGACCGTCAAGTTCACGATCAGTCTCCCGGACGAGGATGTCCACGTCCTCGACGAGTACATCCGGGATCATGGGCTCCG
CGGCCGGTCGGCGGGCGTCCGAGCCGCTGTCCGCCTGCTCGCGGCAGCCGGACTCGAACGGGACTATGCCACGGCCTTTG
CCGAGGTCGACACGGACGACGCGTGGGACGTCACCACGGCCGACACCGAATGA

Structures

Toxin

• AlphaFold DB: A0A098BGQ9