Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1523226..1523845 Replicon chromosome
Accession NZ_CP029137
Organism Klebsiella pneumoniae strain AR376

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CSC13_RS09185 Protein ID WP_002892050.1
Coordinates 1523226..1523444 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag CSC13_RS09190 Protein ID WP_002892066.1
Coordinates 1523471..1523845 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC13_RS09155 1519273..1519536 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
CSC13_RS09160 1519536..1519676 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
CSC13_RS09165 1519673..1520371 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
CSC13_RS09170 1520472..1521923 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
CSC13_RS09175 1521898..1522368 - 471 WP_002892026.1 YlaC family protein -
CSC13_RS29050 1522389..1522529 + 141 WP_004147370.1 hypothetical protein -
CSC13_RS09180 1522501..1523067 - 567 WP_002892030.1 maltose O-acetyltransferase -
CSC13_RS09185 1523226..1523444 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CSC13_RS09190 1523471..1523845 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
CSC13_RS09195 1524331..1527477 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
CSC13_RS09200 1527500..1528693 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T105033 WP_002892050.1 NZ_CP029137:c1523444-1523226 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T105033 NZ_CP029137:c1523444-1523226 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT105033 WP_002892066.1 NZ_CP029137:c1523845-1523471 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT105033 NZ_CP029137:c1523845-1523471 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References