Detailed information of TA system TA105026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 72010..72746
• Replicon: plasmid unnamed2
• Accession: NZ_CP029136
• Organism: Klebsiella pneumoniae strain AR376

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: CSC13_RS00880
• Protein ID: WP_003026803.1
• Coordinates: 72010..72492 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: CSC13_RS00885
• Protein ID: WP_003026799.1
• Coordinates: 72480..72746 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC13_RS00865	67647..68609	+	963	WP_004152113.1	M48 family metalloprotease	-
CSC13_RS00870	68697..69951	+	1255	Protein_66	IS3 family transposase	-
CSC13_RS00875	70452..71798	-	1347	WP_020314316.1	ISNCY family transposase	-
CSC13_RS00880	72010..72492	-	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
CSC13_RS00885	72480..72746	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
CSC13_RS00890	72922..73176	-	255	WP_004152108.1	hypothetical protein	-
CSC13_RS00895	73252..73509	-	258	WP_004152107.1	hypothetical protein	-
CSC13_RS00900	73558..73761	-	204	WP_004152106.1	hemolysin expression modulator Hha	-
CSC13_RS00905	73795..74163	-	369	WP_004152105.1	hypothetical protein	-
CSC13_RS00910	74207..74701	-	495	WP_004152104.1	DNA-binding protein	-
CSC13_RS00915	74732..75307	-	576	WP_004152103.1	hypothetical protein	-
CSC13_RS00920	75295..75564	-	270	WP_004152102.1	hypothetical protein	-
CSC13_RS00925	75922..76272	+	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
CSC13_RS00930	76322..76684	+	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..190416	190416

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T105026 WP_003026803.1 NZ_CP029136:c72492-72010 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T105026 NZ_CP029136:c72492-72010 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT105026 WP_003026799.1 NZ_CP029136:c72746-72480 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT105026 NZ_CP029136:c72746-72480 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66