Detailed information of TA system TA105008

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 2405637..2406350
• Replicon: chromosome
• Accession: NZ_CP029128
• Organism: Klebsiella oxytoca strain AR380

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: CSC17_RS11890
• Protein ID: WP_086530360.1
• Coordinates: 2405637..2405975 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CSC17_RS11895
• Protein ID: WP_086530359.1
• Coordinates: 2406000..2406350 (-)
• Length: 117 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC17_RS11855	2401180..2401503	+	324	WP_049102104.1	endoribonuclease SymE	-
CSC17_RS11860	2401662..2402096	+	435	WP_108704252.1	VOC family protein	-
CSC17_RS11865	2402250..2402993	+	744	WP_108704253.1	hypothetical protein	-
CSC17_RS11870	2403172..2403969	-	798	WP_108704254.1	helix-turn-helix transcriptional regulator	-
CSC17_RS11875	2404085..2404360	-	276	WP_108704255.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CSC17_RS11880	2404364..2404612	-	249	WP_000535213.1	ribbon-helix-helix domain-containing protein	-
CSC17_RS11885	2404689..2405522	-	834	WP_108705043.1	DUF4942 domain-containing protein	-
CSC17_RS11890	2405637..2405975	-	339	WP_086530360.1	TA system toxin CbtA family protein	Toxin
CSC17_RS11895	2406000..2406350	-	351	WP_086530359.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CSC17_RS11900	2406371..2406592	-	222	WP_086530358.1	DUF987 domain-containing protein	-
CSC17_RS11905	2406606..2407103	-	498	WP_108704256.1	DNA repair protein RadC	-
CSC17_RS11910	2407115..2407558	-	444	WP_046876932.1	antirestriction protein	-
CSC17_RS11915	2407638..2407886	-	249	WP_108704257.1	DUF905 domain-containing protein	-
CSC17_RS11920	2407892..2408716	-	825	WP_108704258.1	DUF945 domain-containing protein	-
CSC17_RS11925	2409257..2409889	+	633	WP_072024984.1	inovirus Gp2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2401180..2419462	18282

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (15-115)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12740.70 Da
• Isoelectric Point: 9.6257

>T105008 WP_086530360.1 NZ_CP029128:c2405975-2405637 [Klebsiella oxytoca]
MKTLPANKRAAKPCPSPVAVWQMLLTRLLEQHYGLTLSDTPFNDESIIQEHINAGITLADAVNFLVEKYELVRIDRKGFS
RQEQSPYLRAVDILQARQATGLLRQSHNLLTR

Nucleotide

• Length: 339 bp

>T105008 NZ_CP029128:c2405975-2405637 [Klebsiella oxytoca]
ATGAAAACTCTTCCTGCAAATAAGCGGGCGGCAAAGCCATGCCCGTCGCCTGTGGCCGTTTGGCAGATGTTACTTACCCG
TCTACTTGAGCAGCACTATGGCCTGACCCTTAGCGATACTCCATTCAATGATGAAAGTATTATTCAGGAACATATCAACG
CCGGTATCACACTAGCCGATGCCGTTAACTTTCTGGTGGAAAAGTACGAACTCGTGCGCATCGATCGCAAGGGATTTAGC
AGGCAGGAACAATCGCCTTATCTTCGGGCTGTAGATATTTTGCAGGCGCGACAGGCAACTGGTCTGTTACGACAAAGCCA
TAATCTTTTGACACGCTGA

Antitoxin

Protein

• Length: 117 a.a.
• Molecular weight: 13287.06 Da
• Isoelectric Point: 6.3932

>AT105008 WP_086530359.1 NZ_CP029128:c2406350-2406000 [Klebsiella oxytoca]
MLSKNMTVAHDIAEPWWGLRRDFSPCFGARLVQEGNQLHYLADRASFNGQFSDADLHHLDKTFPVLMKHLELMLTSGEIN
PRHQHCVTLYHNELTCEADTLGSCGYVYLAIYPTQH

Nucleotide

• Length: 351 bp

>AT105008 NZ_CP029128:c2406350-2406000 [Klebsiella oxytoca]
ATGCTGAGTAAAAACATGACCGTAGCTCATGATATTGCTGAACCGTGGTGGGGACTCAGACGCGATTTCTCACCGTGTTT
TGGTGCCCGCCTGGTGCAGGAAGGCAACCAACTGCATTACCTGGCTGACCGCGCCAGTTTTAACGGTCAGTTCAGCGATG
CGGATTTACATCATCTCGACAAGACTTTTCCAGTACTGATGAAACATCTGGAACTGATGCTCACCAGCGGTGAGATTAAT
CCACGGCACCAGCACTGCGTTACGCTGTATCACAACGAACTGACCTGTGAAGCGGATACGCTTGGTAGCTGTGGCTACGT
TTATCTCGCTATCTATCCCACCCAACACTAA