Detailed information of TA system TA105005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1624616..1625235
• Replicon: chromosome
• Accession: NZ_CP029128
• Organism: Klebsiella oxytoca strain AR380

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: H3N9D8
• Locus tag: CSC17_RS08235
• Protein ID: WP_004099646.1
• Coordinates: 1625017..1625235 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: CSC17_RS08230
• Protein ID: WP_108704142.1
• Coordinates: 1624616..1624990 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC17_RS08220	1619772..1620965	+	1194	WP_004111040.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
CSC17_RS08225	1620988..1624134	+	3147	WP_004099650.1	multidrug efflux RND transporter permease subunit	-
CSC17_RS08230	1624616..1624990	+	375	WP_108704142.1	Hha toxicity modulator TomB	Antitoxin
CSC17_RS08235	1625017..1625235	+	219	WP_004099646.1	hemolysin expression modulator Hha	Toxin
CSC17_RS08240	1625396..1625962	+	567	WP_023320460.1	maltose O-acetyltransferase	-
CSC17_RS08245	1626099..1626569	+	471	WP_004111038.1	YlaC family protein	-
CSC17_RS08250	1626544..1627998	-	1455	WP_070557208.1	PLP-dependent aminotransferase family protein	-
CSC17_RS08255	1628101..1628799	+	699	WP_004099639.1	GNAT family N-acetyltransferase	-
CSC17_RS08260	1628796..1628936	-	141	WP_003859006.1	type B 50S ribosomal protein L36	-
CSC17_RS08265	1628936..1629199	-	264	WP_004099638.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8640.05 Da
• Isoelectric Point: 8.9008

>T105005 WP_004099646.1 NZ_CP029128:1625017-1625235 [Klebsiella oxytoca]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Nucleotide

• Length: 219 bp

>T105005 NZ_CP029128:1625017-1625235 [Klebsiella oxytoca]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGCTTACGACGTTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCTGATAATGAACTGGCGGTATTTTACTCAGCAGCCGATCATCGTCTTGCTGAAC
TGACGATGAATAAACTGTATGACAAGATCCCGCCATCGGTCTGGAAATTCATCCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14349.14 Da
• Isoelectric Point: 4.8989

>AT105005 WP_108704142.1 NZ_CP029128:1624616-1624990 [Klebsiella oxytoca]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDIKLVTQLDEYL
DDTFVLFSNYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT105005 NZ_CP029128:1624616-1624990 [Klebsiella oxytoca]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTTAATGACCCAACCTCTGCTGTCAATCTTCAGCTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCACTTAATTATAAAATTAAGTATGCTGAGGATATTAAGCTGGTGACTCAGCTTGACGAATATCTG
GATGACACTTTCGTGTTGTTCAGCAACTACGGCATTAACACTGCAGATCTGCAGAAATGGCGTAAATCAGGCAACAGGTT
ATTCCGCTGCTTTGTGAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A0H3H713