Detailed information of TA system TA105001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 3492797..3493393
• Replicon: chromosome
• Accession: NZ_CP029126
• Organism: Enterobacter hormaechei strain AR432

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A7G3EX93
• Locus tag: CSC19_RS17265
• Protein ID: WP_015570164.1
• Coordinates: 3493091..3493393 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A927HL08
• Locus tag: CSC19_RS17260
• Protein ID: WP_023295755.1
• Coordinates: 3492797..3493084 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC19_RS17255	3491169..3492800	+	1632	WP_003860350.1	Na/Pi cotransporter family protein	-
CSC19_RS17260	3492797..3493084	-	288	WP_023295755.1	putative addiction module antidote protein	Antitoxin
CSC19_RS17265	3493091..3493393	-	303	WP_015570164.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CSC19_RS17270	3493591..3494463	+	873	WP_003860347.1	23S rRNA pseudouridine(2604) synthase RluF	-
CSC19_RS17275	3494464..3494736	-	273	WP_017382612.1	DUF3811 domain-containing protein	-
CSC19_RS17280	3494787..3495731	-	945	WP_015570166.1	ketopantoate/pantoate/pantothenate transporter PanS	-
CSC19_RS17285	3495825..3497174	-	1350	WP_006810501.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11425.18 Da
• Isoelectric Point: 10.3736

>T105001 WP_015570164.1 NZ_CP029126:c3493393-3493091 [Enterobacter hormaechei]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRIYFKDQGNSIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T105001 NZ_CP029126:c3493393-3493091 [Enterobacter hormaechei]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGGATCTATTTTAAGGACCAGGGCAATAGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10101.59 Da
• Isoelectric Point: 5.7807

>AT105001 WP_023295755.1 NZ_CP029126:c3493084-3492797 [Enterobacter hormaechei]
MHKLTPYDPANALLDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Nucleotide

• Length: 288 bp

>AT105001 NZ_CP029126:c3493084-3492797 [Enterobacter hormaechei]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGCTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Structures

Toxin

• AlphaFold DB: A0A7G3EX93