Detailed information of TA system TA104987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 74454..75251
• Replicon: chromosome
• Accession: NZ_CP029126
• Organism: Enterobacter hormaechei strain AR432

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: CSC19_RS00420
• Protein ID: WP_045141441.1
• Coordinates: 74730..75251 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: F5RT92
• Locus tag: CSC19_RS00415
• Protein ID: WP_001303459.1
• Coordinates: 74454..74723 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC19_RS00380	69994..70353	+	360	WP_017384779.1	helix-turn-helix transcriptional regulator	-
CSC19_RS00385	70363..70527	-	165	WP_072044142.1	helix-turn-helix transcriptional regulator	-
CSC19_RS00390	70680..71921	+	1242	WP_017693667.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
CSC19_RS00395	71954..72181	-	228	WP_006809324.1	YccJ family protein	-
CSC19_RS00400	72200..72796	-	597	WP_006809323.1	NAD(P)H:quinone oxidoreductase	-
CSC19_RS00405	73188..73358	+	171	WP_001273664.1	general stress protein	-
CSC19_RS00410	73475..74380	+	906	WP_045141440.1	DMT family transporter	-
CSC19_RS00415	74454..74723	+	270	WP_001303459.1	DUF1778 domain-containing protein	Antitoxin
CSC19_RS00420	74730..75251	+	522	WP_045141441.1	GNAT family N-acetyltransferase	Toxin
CSC19_RS00425	75310..76632	-	1323	WP_045141442.1	pyrimidine utilization transport protein G	-
CSC19_RS00430	76654..77145	-	492	WP_045141443.1	pyrimidine utilization flavin reductase protein F	-
CSC19_RS00435	77158..77748	-	591	WP_062934089.1	malonic semialdehyde reductase	-
CSC19_RS00440	77758..78558	-	801	WP_108720685.1	pyrimidine utilization protein D	-
CSC19_RS00445	78566..78952	-	387	WP_015570870.1	pyrimidine utilization protein C	-
CSC19_RS00450	78964..79653	-	690	WP_108720686.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-88)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19407.16 Da
• Isoelectric Point: 7.4318

>T104987 WP_045141441.1 NZ_CP029126:74730-75251 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLVRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTALVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Nucleotide

• Length: 522 bp

>T104987 NZ_CP029126:74730-75251 [Enterobacter hormaechei]
GTGGCAAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTACGATTTCGGCGATTTCGATTGTGGTGAACCCTCGCT
AAATGCCTTCCTTACCGAGCACCTGGTACGTCAGCACGGCGGGCGTATTTTACGCGGCTACCTGCTTAAAGAACGCGACC
ATCCGCGTGTCCTGGGCTATTACACACTCTCGGGAAGTTGCTTTGAAAAAGCGATGCTTCCTTCAAAGACTCAGCAGCGC
CGCATCCCATACAGCAACGTCCCCAGCGTGACGCTGGGGCGTCTGGCTGTCCATAAAGAACTCCAGGGAAACGAGTGGGG
AACAGCGCTTGTCACACACGCTATGCGCGTTGTTTATCTGGCGTCGCAGGCTGTGGGCGTCCACGGAATTTTTGTGGATG
CGCTTAACGAACGGGCCAAACGATTTTATCTGAAGCTGGGGTTTATCCCGCTGGCAGGTGAAAACAGCAGTTCGCTGTTC
TTCCCCACGCAATCTATAGAACGACTGTTTGAGCAGGCGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10248.70 Da
• Isoelectric Point: 5.8836

>AT104987 WP_001303459.1 NZ_CP029126:74454-74723 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT104987 NZ_CP029126:74454-74723 [Enterobacter hormaechei]
ATGCCAGCACTCAAAAAACAGCGTATCGATCTTCGCCTGTCGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACCAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCCGAAGTCATCGAACAGCATCGGCGTC
TCGTATTAAACGAAGCCTCATGGAATGCCGTTATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7D7BCZ2