Detailed information of TA system TA104973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1601796..1602421
• Replicon: chromosome
• Accession: NZ_CP029122
• Organism: Escherichia coli strain AR434

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CSC21_RS08175
• Protein ID: WP_000911330.1
• Coordinates: 1602023..1602421 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: CSC21_RS08170
• Protein ID: WP_000450524.1
• Coordinates: 1601796..1602023 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC21_RS08145	1597599..1598069	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
CSC21_RS08150	1598069..1598641	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
CSC21_RS08155	1598787..1599665	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CSC21_RS08160	1599682..1600716	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
CSC21_RS08165	1600929..1601642	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CSC21_RS08170	1601796..1602023	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CSC21_RS08175	1602023..1602421	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CSC21_RS08180	1602568..1603431	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
CSC21_RS08185	1603446..1605461	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
CSC21_RS08190	1605535..1606233	+	699	WP_000679823.1	esterase	-
CSC21_RS08195	1606343..1606543	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T104973 WP_000911330.1 NZ_CP029122:1602023-1602421 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T104973 NZ_CP029122:1602023-1602421 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT104973 WP_000450524.1 NZ_CP029122:1601796-1602023 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT104973 NZ_CP029122:1601796-1602023 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33