Detailed information of TA system TA104961

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 68214..68815
• Replicon: plasmid unnamed4
• Accession: NZ_CP029117
• Organism: Escherichia coli strain AR435

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: CSC22_RS26235
• Protein ID: WP_001216045.1
• Coordinates: 68435..68815 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: CSC22_RS26230
• Protein ID: WP_001190712.1
• Coordinates: 68214..68435 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC22_RS26180	63400..63663	+	264	WP_000224220.1	hypothetical protein	-
CSC22_RS26185	63674..64273	+	600	WP_053903357.1	DUF551 domain-containing protein	-
CSC22_RS26190	64356..64589	+	234	WP_000516537.1	hypothetical protein	-
CSC22_RS26195	64768..65061	+	294	WP_001677496.1	hypothetical protein	-
CSC22_RS26200	65068..65442	+	375	WP_023154376.1	hypothetical protein	-
CSC22_RS26205	65424..66356	+	933	WP_077780118.1	hypothetical protein	-
CSC22_RS26210	66353..66715	+	363	WP_001261544.1	hypothetical protein	-
CSC22_RS28690	66790..66927	-	138	WP_000123562.1	hypothetical protein	-
CSC22_RS26220	67377..67628	+	252	WP_001377386.1	DNA polymerase III subunit theta	-
CSC22_RS26225	67752..68141	+	390	WP_000506726.1	DNA repair protein	-
CSC22_RS26230	68214..68435	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CSC22_RS26235	68435..68815	+	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
CSC22_RS26240	68820..68999	+	180	WP_000113019.1	hypothetical protein	-
CSC22_RS26245	69027..70070	+	1044	WP_000648823.1	DUF968 domain-containing protein	-
CSC22_RS26250	70159..70611	+	453	WP_001369802.1	hypothetical protein	-
CSC22_RS26255	70698..71891	+	1194	WP_000219605.1	terminase	-
CSC22_RS26260	71891..73375	+	1485	WP_000124155.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..120846	120846

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T104961 WP_001216045.1 NZ_CP029117:68435-68815 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T104961 NZ_CP029117:68435-68815 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT104961 WP_001190712.1 NZ_CP029117:68214-68435 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT104961 NZ_CP029117:68214-68435 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6