Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3903337..3903991 Replicon chromosome
Accession NZ_CP029115
Organism Escherichia coli strain AR435

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag CSC22_RS20730 Protein ID WP_000244777.1
Coordinates 3903337..3903744 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CSC22_RS20735 Protein ID WP_000354046.1
Coordinates 3903725..3903991 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC22_RS20710 3899294..3901027 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
CSC22_RS20715 3901033..3901743 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CSC22_RS20720 3901768..3902664 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CSC22_RS20725 3902776..3903297 + 522 WP_001055874.1 flavodoxin FldB -
CSC22_RS20730 3903337..3903744 - 408 WP_000244777.1 toxin CptA Toxin
CSC22_RS20735 3903725..3903991 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CSC22_RS20740 3904234..3905214 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
CSC22_RS20745 3905410..3906069 - 660 WP_000250274.1 hemolysin III family protein -
CSC22_RS20750 3906233..3906544 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
CSC22_RS20755 3906589..3908022 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
CSC22_RS20760 3908079..3908822 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T104956 WP_000244777.1 NZ_CP029115:c3903744-3903337 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T104956 NZ_CP029115:c3903744-3903337 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT104956 WP_000354046.1 NZ_CP029115:c3903991-3903725 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT104956 NZ_CP029115:c3903991-3903725 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References