Detailed information of TA system TA104953

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2958750..2959585
• Replicon: chromosome
• Accession: NZ_CP029115
• Organism: Escherichia coli strain AR435

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1X9TM58
• Locus tag: CSC22_RS16155
• Protein ID: WP_000854761.1
• Coordinates: 2959208..2959585 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: CSC22_RS16150
• Protein ID: WP_001295723.1
• Coordinates: 2958750..2959118 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC22_RS16115	2955859..2956017	+	159	WP_001323397.1	DUF905 family protein	-
CSC22_RS16125	2956172..2956990	+	819	WP_001234729.1	DUF945 domain-containing protein	-
CSC22_RS16135	2957332..2957805	+	474	WP_000855059.1	antirestriction protein	-
CSC22_RS16140	2957821..2958297	+	477	WP_001424026.1	RadC family protein	-
CSC22_RS16145	2958366..2958587	+	222	WP_000692345.1	DUF987 domain-containing protein	-
CSC22_RS16150	2958750..2959118	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CSC22_RS16155	2959208..2959585	+	378	WP_000854761.1	TA system toxin CbtA family protein	Toxin
CSC22_RS16160	2959582..2959784	+	203	Protein_2782	hypothetical protein	-
CSC22_RS16165	2959797..2959910	+	114	WP_001161660.1	DUF957 domain-containing protein	-
CSC22_RS16170	2960345..2961880	-	1536	WP_001333339.1	IS66-like element ISEc22 family transposase	-
CSC22_RS16175	2961929..2962276	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
CSC22_RS16180	2962273..2962677	-	405	WP_000839179.1	IS66 family insertion sequence hypothetical protein	-
CSC22_RS16185	2963171..2963560	+	390	WP_001445118.1	IS110 family transposase	-
CSC22_RS16195	2964014..2964196	+	183	WP_001016348.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14205.25 Da
• Isoelectric Point: 7.3249

>T104953 WP_000854761.1 NZ_CP029115:2959208-2959585 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T104953 NZ_CP029115:2959208-2959585 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT104953 WP_001295723.1 NZ_CP029115:2958750-2959118 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT104953 NZ_CP029115:2958750-2959118 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TM58

Antitoxin

• AlphaFold DB: S1NM52